Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection

	Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection
	Tang, Chunlan; Wang, Li; Cheng, Mengchun; Zhang, Xiaozhe; Liu, Xiaoyan; Xiao, Hongbin
刊名	journal of chromatography b-analytical technologies in the biomedical and life sciences
	2014-12-15
卷号	973 页码:104-109
关键词	Parishin UHPLC-FLD Metabolites Pharmacokinetics
英文摘要	a simple, rapid and sensitive ultra high performance liquid chromatography with fluorescence detection (uhplc-fld) method was developed and validated for quantification of parishin and its metabolites in rats. plasma samples were prepared by protein precipitation and then analyzed using uhplc-fld system. repeated optimization showed that parishin and its metabolites, including gastrodin, p-hydroxybenzyl alcohol, parishin b and parishin c, could be sensitively detected based on the autofluorescence when excitation and emission wavelengths were set at 225 nm and 295 nm, respectively. the limit of detections (lods) of gas, hba, pb, pc and pa reached 0.6, 0.8, 1, 1 and 1 ng/ml, respectively. the linearity for all targets was within the range 2.5-5000 ng/ml and the correlation coefficient (r(2)) was larger than 0.999. importantly, our method was almost free from matrix effects and the recoveries were higher than 80%. additionally, our method also had high precision and accuracy for all analytes, presenting rsds and res within +/- 6% and +/- 14%, respectively. finally, the validated uhplc-fld method was successfully applied for studying the pharmacokinetics of parishin following intragastrically administration in rats. (c) 2014 elsevier b.v. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	gastrodia-elata-bl. ; pharmacokinetics ; identification ; microdialysis ; constituents ; rhizomes
收录类别	SCI
语种	英语
WOS记录号	WOS:000345878800015
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/145414]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Tang, Chunlan,Wang, Li,Cheng, Mengchun,et al. Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2014,973:104-109.
APA	Tang, Chunlan,Wang, Li,Cheng, Mengchun,Zhang, Xiaozhe,Liu, Xiaoyan,&Xiao, Hongbin.(2014).Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection.journal of chromatography b-analytical technologies in the biomedical and life sciences,973,104-109.
MLA	Tang, Chunlan,et al."Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection".journal of chromatography b-analytical technologies in the biomedical and life sciences 973(2014):104-109.