Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction

	Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction
	Cao, Liwei 1; Zhang, Ye 2; Chen, Linlin 2; Shen, Aijin 1; Zhang, Xingwang 3; Ren, Shifang 3; Gu, Jianxin 3; Yu, Long 1; Liang, Xinmiao 1
刊名	analyst
	2014-09-21
卷号	139 期号:18 页码:4538-4546
通讯作者	于龙 ; 梁鑫淼
英文摘要	expression levels of n-linked glycans derived from human serum glycoproteins have been shown to change during the progression of many diseases. generally, n-glycans released from human serum proteins co-exist with endogenous serum peptides, salts, and other contaminants. effective removal of these contaminants is essential to obtain the glycan profile of human serum proteins. here, we developed a sample preparation method for mass spectrometry (ms) analysis of n-linked glycans derived from human serum glycoproteins based on a zwitterionic hydrophilic material named click te-cys. the high hydrophilicity of click te-cys, resulting from its unique surface structure and charge distribution, facilitated removal of co-existing salts and endogenous serum peptides. furthermore, the present enrichment approach was handled in parallel, thus saving time. using this method, a total of 47 unique n-glycans released from human serum proteins were identified. the intrabatch and interbatch coefficients of variation for the 47 n-linked glycans were 8.57% +/- 0.96% and 9.22% +/- 1.03%, respectively. these results demonstrate that the present method is suitable for fast purification of n-linked glycans derived from human serum glycoproteins, and has potential for clinical application.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	ordered mesoporous carbon ; human plasma proteome ; linked glycans ; selective enrichment ; ovarian-cancer ; glycomics ; glycosylation ; glycopeptides ; deglycosylation ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000340755000018
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144355]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China 3.Fudan Univ, Dept Biochem & Mol Biol, Key Lab Glycoconjugate Res, Minist Publ Hlth,Sch Basic Med Sci, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Cao, Liwei,Zhang, Ye,Chen, Linlin,et al. Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction[J]. analyst,2014,139(18):4538-4546.
APA	Cao, Liwei.,Zhang, Ye.,Chen, Linlin.,Shen, Aijin.,Zhang, Xingwang.,...&Liang, Xinmiao.(2014).Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction.analyst,139(18),4538-4546.
MLA	Cao, Liwei,et al."Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction".analyst 139.18(2014):4538-4546.