Current state-of-the-art of nontargeted metabolomics based on liquid chromatography-mass spectrometry with special emphasis in clinical applications

	Current state-of-the-art of nontargeted metabolomics based on liquid chromatography-mass spectrometry with special emphasis in clinical applications
	Yin, Peiyuan; Xu, Guowang
刊名	journal of chromatography a
	2014-12-29
卷号	1374 期号:1 页码:1-13
关键词	Metabolomics Metabolic profiling LC-MS Metabolite coverage Pseudo-targeted method
通讯作者	许国旺
英文摘要	metabolomics, as a part of systems biology, has been widely applied in different fields of life science by studying the endogenous metabolites. the development and applications of liquid chromatography (lc) coupled with high resolution mass spectrometry (ms) greatly improve the achievable data quality in non-targeted metabolic profiling. however, there are still some emerging challenges to be covered in lc-ms based metabolomics. here, recent approaches about sample collection and preparation, instrumental analysis, and data handling of lc-ms based metabolomics are summarized, especially in the analysis of clinical samples. emphasis is put on the improvement of analytical techniques including the combination of different lc columns, isotope coded derivatization methods, pseudo-targeted lc-ms method, new data analysis algorithms and structural identification of important metabolites. (c) 2014 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	solid-phase microextraction ; ms-based metabolomics ; gestational diabetes-mellitus ; functional genomics tool ; global systems biology ; human blood-plasma ; tert-butyl ether ; lc-ms ; metabolite identification ; sample preparation
收录类别	SCI
语种	英语
WOS记录号	WOS:000347605000001
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://cas-ir.dicp.ac.cn/handle/321008/144198]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yin, Peiyuan,Xu, Guowang. Current state-of-the-art of nontargeted metabolomics based on liquid chromatography-mass spectrometry with special emphasis in clinical applications[J]. journal of chromatography a,2014,1374(1):1-13.
APA	Yin, Peiyuan,&Xu, Guowang.(2014).Current state-of-the-art of nontargeted metabolomics based on liquid chromatography-mass spectrometry with special emphasis in clinical applications.journal of chromatography a,1374(1),1-13.
MLA	Yin, Peiyuan,et al."Current state-of-the-art of nontargeted metabolomics based on liquid chromatography-mass spectrometry with special emphasis in clinical applications".journal of chromatography a 1374.1(2014):1-13.