Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters

	Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters
	Teng,Ye; Jia,Xiaofang; Li,Jing; Wang,Erkang
刊名	analytical chemistry
	2015
卷号	87 期号:9 页码:4897-4902
关键词	TURN-ON METAL NANOCLUSTERS AU NANOCLUSTERS LIVE CELLS NANOPARTICLES PROBE SENSOR BIOSENSOR ACID SENSITIVITY
通讯作者	han,dx
英文摘要	in this work, a sensitive and selective ratiometric fluorescence sensing platform was built for the detection of tyrosinase (tyr) activity and dopamine (da) using glutathione (gsh) protected gold nanoclusters (au ncs) as probes. upon excitation at 350 nm, au ncs displayed an intense red emission, which could be effectively quenched by quinones. tyr, a typical polyphenol oxidase, can catalyze the oxidization of da to o-quinone and therefore quenched the fluorescence of au ncs. moreover, the reaction of tyr and da gave rise to an emission band at 400 nm, which increased in a tyr/da-concentration-dependent manner. the ratiometric signal variations were utilized for facile, sensitive, and selective detection of tyr activity and da. a linear range was obtained from 0.006-3.6 unit ml(-1) of tyr activity, while the linear range for detection of da was 1.0 nm to 1.0 mm. additionally, it constructed a useful platform for tyr inhibitor screening in biomedical research.
收录类别	SCI
语种	英语
WOS记录号	WOS:000354153600044
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64839]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Teng,Ye,Jia,Xiaofang,Li,Jing,et al. Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters[J]. analytical chemistry,2015,87(9):4897-4902.
APA	Teng,Ye,Jia,Xiaofang,Li,Jing,&Wang,Erkang.(2015).Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters.analytical chemistry,87(9),4897-4902.
MLA	Teng,Ye,et al."Ratiometric Fluorescence Detection of Tyrosinase Activity and Dopamine Using Thiolate-Protected Gold Nanoclusters".analytical chemistry 87.9(2015):4897-4902.