Codelivery of Antitumor Drug and Gene by a pH-Sensitive Charge-Conversion System

	Codelivery of Antitumor Drug and Gene by a pH-Sensitive Charge-Conversion System
	Guan,Xiuwen; Li,Yanhui; Jiao,Zixue; Lin,Lin; Chen,Jie; Guo,Zhaopei; Tian,Huayu; Chen,Xuesi
刊名	acs applied materials & interfaces
	2015
卷号	7 期号:5 页码:3207-3215
关键词	LOW-MOLECULAR-WEIGHT BREAST-TUMOR CELLS IN-VIVO ANTICANCER DRUGS CO-DELIVERY P53 DOXORUBICIN DNA THERAPY POLYETHYLENIMINE
通讯作者	li,yh
英文摘要	in the present study, a gene and drug codelivery system was developed by electrostatic binding of polyethylenimine-poly(l-lysine)-poly(l-glutamic acid) (pelg), polyethylenimine (pei), cis-aconityl-doxorubicin (cad), and dna. zeta potential and drug release analysis confirmed the ph-responsive charge conversion and acid-sensitive drug release functional properties of the pelg/pei/(dna+cad) system. gel retardation assay and transfection experiment showed the codelivery system had effective dna binding ability and good transfection efficiency on hepg2 cells. the therapeutic gene p53 was further employed to study its combinational effects with cad. cytotoxicity assay showed the half inhibitory concentration (ic50) of the pelg/pei/(p53+cad) codelivery system was lower than that of the gene or the drug delivery system. confocal laser scanning microscopy (clsm) showed that the drug and gene could be delivered into the cells simultaneously. a significant increase of p53 gene expression was achieved after hepg2 cells treated by pelg/pei/(p53+cad) codelivery system. the apoptosis experiment indicated clearly that the codelivery system could lead an effective apoptosis on tumor cells, which was beneficial for the treatment of cancer. the biodistribution and tumor accumulation of the codelivery system was explored via in vivo imaging in subcutaneous xenograft and in situ tumor models. the tumor and some major organs were excised and imaged, and the results showed that the codelivery system can accumulate efficiently in tumor for both tumor models. it can be suggested from the above results that the pelg/pei/(dna+cad) codelivery system will have great potential applications in cancer therapy.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64742]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Guan,Xiuwen,Li,Yanhui,Jiao,Zixue,et al. Codelivery of Antitumor Drug and Gene by a pH-Sensitive Charge-Conversion System[J]. acs applied materials & interfaces,2015,7(5):3207-3215.
APA	Guan,Xiuwen.,Li,Yanhui.,Jiao,Zixue.,Lin,Lin.,Chen,Jie.,...&Chen,Xuesi.(2015).Codelivery of Antitumor Drug and Gene by a pH-Sensitive Charge-Conversion System.acs applied materials & interfaces,7(5),3207-3215.
MLA	Guan,Xiuwen,et al."Codelivery of Antitumor Drug and Gene by a pH-Sensitive Charge-Conversion System".acs applied materials & interfaces 7.5(2015):3207-3215.