Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus

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	Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus
	Jiang, Lixiang 1,2; Luo, Jing 1; Dong, Wenjie 3; Wang, Chengmin 1; Jin, Wen 1; Xia, Yuetong 3; Wang, Haijing 1; Ding, Hua 4; Jiang, Long 3; He, Hongxuan 1
刊名	JOURNAL OF VIROLOGICAL METHODS
	2015-07-01
卷号	219 页码:38-45
关键词	Polydiacetylene H5 influenza virus Biosensor Chromatic immunoassay Detection Tracheal swabs
英文摘要	H5N1 avian influenza has caused serious economic losses as well as posed significant threats to public health, agriculture and wildlife. It is important to develop a rapid, sensitive and specific detection platform suitable for disease surveillance and control. In this study, a highly sensitive, specific and rapid biosensor based on polydiacetylene was developed for detecting H5 influenza virus. The polydiacetylene based biosensor was produced from an optimized ratio of 10,12-pentacosadiynoic acid and 1,2-dimyristoyl-snglycero-3-phosphocholine, with the anti-H5 influenza antibody embedded onto the vesicle surface. The optimized polydiacetylene vesicle could detect H5 influenza virus sensitively with a detection limit of 0.53 copies/mu L, showing a dramatic blue-to-red color change that can be observed directly by the naked eye and recorded by a UV-vis spectrometer. The sensitivity, specificity and accuracy of the biosensor were also evaluated. The sensor could specifically differentiate H5 influenza virus from H3 influenza virus, Newcastle disease virus and porcine reproductive and respiratory syndrome virus. Detection using tracheal swabs was in accord with virus isolation results, and comparable to the RT-PCR method. These results offer the possibility and potential of simple polydiacetylene based bio-analytical method for influenza surveillance. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28070]
专题	化学研究所_胶体、界面与化学热力学实验室
作者单位	1.Chinese Acad Sci, Natl Res Ctr Wildlife Born Dis, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Being Natl Lab Mol Sci, Inst Chem, Beijing 100101, Peoples R China 4.Hangzhou Ctr Dis Control & Prevent, Dept Infect Dis, Hangzhou 310021, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Lixiang,Luo, Jing,Dong, Wenjie,et al. Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus[J]. JOURNAL OF VIROLOGICAL METHODS,2015,219:38-45.
APA	Jiang, Lixiang.,Luo, Jing.,Dong, Wenjie.,Wang, Chengmin.,Jin, Wen.,...&He, Hongxuan.(2015).Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus.JOURNAL OF VIROLOGICAL METHODS,219,38-45.
MLA	Jiang, Lixiang,et al."Development and evaluation of a polydiacetylene based biosensor for the detection of H5 influenza virus".JOURNAL OF VIROLOGICAL METHODS 219(2015):38-45.