Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters

	Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters
	Zhao, Qian 1; Huang, Haowen 1; Zhang, Lingyang 1; Wang, Linqian 2; Zeng, Yunlong 1; Xia, Xiaodong 1; Liu, Fengping 1; Chen, Yi 3
刊名	ANALYTICAL CHEMISTRY
	2016-01-19
卷号	88 期号:2 页码:1412-1418
英文摘要	It is broadly interesting but remains a big challenge to explore nanomaterials-based methods to enable naked-eye observation and determination of ultratrace biomarkers and drugs. In this study, we developed a straightforward and extendable plasmonic nanosensor to enable visually quantitative determination of ultratrace target molecules through combining the use of enzyme-mimetic gold nanoclusters (AuNCs). Starting from sandwiched antibody-antigen (i.e., an analyte)-antibody structure, we conjugated AuNCs on the outer layer antibody to catalyze the decomposition of hydrogen peroxide used to reduce HAuCl4 into gold nanopartilces (AuNPs) for naked eye readout. This strategy is in theory applicable to all immunoreactions available and the protocol proposed to attach AuNCs onto an antibody is suitable to all proteins. The applicability of this type of nanosensor was validated by the determination of various ultratrace analytes such as protein avidin, breast cancer antigen, thyroid hormone, and even methamphetamine (MA), giving a naked-eye-readout limit of detection (LOD), down to 1.0 x 10(-20) M protein avidin, 7.52 x 10(-14) U/mL breast cancer antigen 15-3, 2.0 x 10(-15) mg/mL 3,5,3'-L-triiodothyronine and 2.3 x 10(-18) mg/mL MA. This strategy is thus considered an ultrasensitive way to fabricate plasmonic nanosensors, having wide and invaluable application potential in clinical, biological, and environmental studies, and in food quality control.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/30102]
专题	化学研究所_活体分析化学实验室
作者单位	1.Hunan Univ Sci & Technol, Sch Chem & Chem Engn, QSAR QSPR,Hunan Prov Univ Key Lab, Key Lab Theoret Organ Chem & Funct Mol,Minist Edu, Xiangtan, Peoples R China 2.Cent S Univ, Xiangya Sch Med, Affiliated Canc Hosp, Dept Med Lab,Hunan Canc Hosp, Changsha, Hunan, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Qian,Huang, Haowen,Zhang, Lingyang,et al. Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters[J]. ANALYTICAL CHEMISTRY,2016,88(2):1412-1418.
APA	Zhao, Qian.,Huang, Haowen.,Zhang, Lingyang.,Wang, Linqian.,Zeng, Yunlong.,...&Chen, Yi.(2016).Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters.ANALYTICAL CHEMISTRY,88(2),1412-1418.
MLA	Zhao, Qian,et al."Strategy To Fabricate Naked-Eye Readout Ultrasensitive Plasmonic Nanosensor Based on Enzyme Mimetic Gold Nanoclusters".ANALYTICAL CHEMISTRY 88.2(2016):1412-1418.