In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets

	In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets
	He, Qing 1; Badu-Tawiah, Abraham K.2; Chen, Suming 1,2; Xiong, Caiqiao 1; Liu, Huihui 1; Zhou, Yueming 1; Hou, Jian 1; Zhang, Ning 1; Li, Yafeng 1; Xie, Xiaobo 1
刊名	ANALYTICAL CHEMISTRY
	2015-03-17
卷号	87 期号:6 页码:3144-3148
英文摘要	Molecular ions are generated in induced electrospray ionization, and they can be transported to grounded ambient surfaces in the form of-charged microdroplets. Efficient amide bonds formation between ainines and carboxylic aids were observed inside charged droplets during transfer to the surface. Biomolecules derivatized using this method were self-assembled on a bare gold surface via Au-S bonds under the charged microdroplet environment. Cyclic voltammetric analysis of the self-assembled molecular film showed accelerated protein derivatization With cysteine, which allowed the covalent immobilization of the protein to the gold surface Cytochrome C-functionalized electrodes prepared using the induced dual nanoelectrospray process showed bioactivity toward aqueous solutions of hydrogen peroxide below 50 mu M. In effect, we have developed a. method that allows derivatization of biomolecules and their immobilization at ambient surfaces in a single experimental step.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28433]
专题	化学研究所_活体分析化学实验室
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 USA
推荐引用方式 GB/T 7714	He, Qing,Badu-Tawiah, Abraham K.,Chen, Suming,et al. In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets[J]. ANALYTICAL CHEMISTRY,2015,87(6):3144-3148.
APA	He, Qing.,Badu-Tawiah, Abraham K..,Chen, Suming.,Xiong, Caiqiao.,Liu, Huihui.,...&Nie, Zongxiu.(2015).In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets.ANALYTICAL CHEMISTRY,87(6),3144-3148.
MLA	He, Qing,et al."In Situ Bioconjugation and Ambient Surface Modification Using Reactive Charged Droplets".ANALYTICAL CHEMISTRY 87.6(2015):3144-3148.