In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system

	In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system
	Gu, Feidan 1; Zhou, Xiaoyu 1; Zhu, Xiaocui 1; Zhao, Meiping 1; Hao, Jie 2; Yu, Ping 2; Mao, Lanqun 2
刊名	ANALYST
	2015
卷号	140 期号:11 页码:3814-3819
英文摘要	An on-line and continuous approach was demonstrated for in vivo measurement of bisulfide in rat's brain. A modified droplet-based microfluidic system was constructed, which allowed on-line qualification of the fluorescence responses of the gold nanoparticle-glutathione-fluorescein isothiocyanate probe to the variation of bisulfide in the presence of the cerebral microdialysate background. The on-line method achieved a dynamic working range from 5.0 mu M to 40 mu M and a detection limit of 2.5 mu M. The in vivo bisulfide concentration in the hippocampus of rat's brain was measured under different physiological conditions. The on-line method may facilitate the study of H2S biology by providing a previously unattainable continuous record of H2S variation in living animals. It also provides a practical platform for in vivo and continuous monitoring of other important species in cerebral systems.
收录类别	SCI
语种	英语
公开日期	2016-05-09
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/28065]
专题	化学研究所_活体分析化学实验室
作者单位	1.Peking Univ, Coll Chem & Mol Engn, MOE Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Gu, Feidan,Zhou, Xiaoyu,Zhu, Xiaocui,et al. In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system[J]. ANALYST,2015,140(11):3814-3819.
APA	Gu, Feidan.,Zhou, Xiaoyu.,Zhu, Xiaocui.,Zhao, Meiping.,Hao, Jie.,...&Mao, Lanqun.(2015).In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system.ANALYST,140(11),3814-3819.
MLA	Gu, Feidan,et al."In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system".ANALYST 140.11(2015):3814-3819.