Simultaneous determination of the content of isoquinoline alkaloids in Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids by high-performance liquid chromatography with diode array detection | |
Chen YL(陈亚丽)1,2; Li M(李敏)1; Jianjun Liu3; Qian Yan1; Mei Zhong1; Liu JX(柳军玺)1; Di DL(邸多隆)1; Jinxia Liu4 | |
刊名 | Journal of Separation Science |
2015 | |
卷号 | 38期号:1页码:9-17 |
关键词 | Dicranostigma leptopodum (Maxim) Fedde High-performance liquid chromatography Isocorydine Isoquinoline alkaloids |
ISSN号 | 1615-9306 |
通讯作者 | 柳军玺 |
英文摘要 | A simple and efficient method was developed for the simultaneous determination of eight isoquinoline alkaloids in methanol extracts of Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids of D. leptopodum by high-performance liquid chromatography with diode array detection. The chromatographic conditions were optimized on a SinoChrom ODS-BP column to obtain a good separation of the four types of alkaloid analytes, including two aporphines (isocorydine, corydine), two protopines (protopine and allocryptopine), a morphine (sinoacutine), and three quaternary protoberberine alkaloids (berberrubine, 5-hydroxycoptisine, and berberine). The separation of these alkaloids was significantly affected by the composition of the mobile phase, and particularly by its pH value. Acetonitrile (A) and 0.2% phosphoric acid solution adjusted to pH 6.32 with triethylamine (B) were selected as the mobile phase with a gradient elution. With this method, a new quaternary protoberberine alkaloid was isolated and the two structural isomers (isocorydine and corydine) were baseline separated. The appropriate harvest period for D. leptopodum was also recommended based on our analysis. The method for the effective fraction of the alkaloids of D. leptopodum was optimized under this method with regard to the varying significant pharmacological activities of the alkaloids. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the "Western Light" program;the "Bulid Coalitions of the National Academy of Sciences" program of the Chinese Academy of Sciences;the Science and Technology program of Gansu (1304FKCA062) |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/19478] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
作者单位 | 1.Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, PR China 2.Institute of Medicinal Chemistry, School Pharmacy,Lanzhou University, Lanzhou, PR China 3.University hospital of Gansu Traditional Chinese Medicine, Lanzhou, PR China 4.Institute of Biology, Gansu Academy of Sciences, Lanzhou, PR China |
推荐引用方式 GB/T 7714 | Chen YL,Li M,Jianjun Liu,et al. Simultaneous determination of the content of isoquinoline alkaloids in Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids by high-performance liquid chromatography with diode array detection[J]. Journal of Separation Science,2015,38(1):9-17. |
APA | Chen YL.,Li M.,Jianjun Liu.,Qian Yan.,Mei Zhong.,...&Jinxia Liu.(2015).Simultaneous determination of the content of isoquinoline alkaloids in Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids by high-performance liquid chromatography with diode array detection.Journal of Separation Science,38(1),9-17. |
MLA | Chen YL,et al."Simultaneous determination of the content of isoquinoline alkaloids in Dicranostigma leptopodum (Maxim) Fedde and the effective fractionation of the alkaloids by high-performance liquid chromatography with diode array detection".Journal of Separation Science 38.1(2015):9-17. |
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