Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 朱毅敏团队

	Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment
	Pu, KF(濮科锋); Yuan, LH(原丽华); Chen, LS(陈丽莎); Wang, AX(王安欣); Zhou, X(周旋); Zhang, HL(张海禄); Zhu, YM(朱毅敏)
刊名	CURRENT CANCER DRUG TARGETS
	2015
卷号	15 期号:2 页码:13
关键词	Angiogenesis bacterial surface display cancer diagnosis cancer therapy peptide vascular endothelial growth factor receptor 2 (VEGFR2)
通讯作者	Zhu, YM (朱毅敏)
英文摘要	The signal transduction pathway initiated by vascular endothelial growth factor-vascular endothelial growth factor receptor 2 (VEGF-VEGFR2) plays an important role in the angiogenesis of tumors. The effective antagonists of VEGFR2 would behave as potent drugs for the treatment of malignant cancers. In our study, specific binding peptides with high affinity to VEGFR2 were obtained through bacterial display technology. Conserved motif (FF/YEXWGVK) among those peptide sequences was discovered. One of the selected peptides, VRBP1 (YDGNSFYEMWGVKPASES) was identified by screening the biased bacterial peptide library and its physiochemical feature was further characterized. The results of surface plasmon resonance (SPR) assay indicated that the dissociation constant (K-D) value of VRBP1 was 228.3 nM and this peptide competed with VEGF binding to VEGFR2. Particles conjugated with VRBP1 could recognize the human umbilical vein endothelial cells (HUVEC) which express VEGFR2 on the surface. Further therapeutic effect of VRBP1 was examined by in vivo experiments. VRBP1 could result in a significant decrease in tumor size of H460 xenografts. The results from the immunohistochemical assay showed that CD31 positive signals in VRBP1-treated group were fewer than those in the control ones. These data highlighted the potential of VEGFR2-binding peptides as effective molecules for cancer diagnosis and therapy.
收录类别	SCI
语种	英语
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/3482]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_朱毅敏团队
推荐引用方式 GB/T 7714	Pu, KF,Yuan, LH,Chen, LS,et al. Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment[J]. CURRENT CANCER DRUG TARGETS,2015,15(2):13.
APA	Pu, KF.,Yuan, LH.,Chen, LS.,Wang, AX.,Zhou, X.,...&Zhu, YM.(2015).Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment.CURRENT CANCER DRUG TARGETS,15(2),13.
MLA	Pu, KF,et al."Identification of VEGFR2-Binding Peptides Using High Throughput Bacterial Display Methods and Functional Assessment".CURRENT CANCER DRUG TARGETS 15.2(2015):13.