Identification of stem-like cells in non-small cell lung cancer cells with specific peptides

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 朱毅敏团队

	Identification of stem-like cells in non-small cell lung cancer cells with specific peptides
	Zhu YM(朱毅敏)
刊名	CANCER LETTERS
	2014-08-28
卷号	351 期号:1 页码:100-107
关键词	Cancer stem-like cells Tumor spheres Peptide MicroRNA Bacterial surface display
通讯作者	朱毅敏
英文摘要	Recent studies indicate that tumor maintenance, metastasis and drug-resistance are mainly conducted by a small subset of cancer cells which are termed cancer stem cells (CSCs) or cancer stem-like cells (CSLCs). Successful identification of CSCs/CSLCs might lead to discovery of the novel and effective therapeutic targets for cancers. In our study, lung CSCs/CSLCs were enriched by sphere-forming assay. Screening and selection of specific binding peptides for lung CSCs/CSLCs were performed with bacterial surface display method. Selected peptide named HCBP-1 exhibited highest specific binding capability as examined by flow cytometry and fluorescence microscopy. Drug-resistant lung CSCs/CSLCs might be characterized with HCBP-1 peptide and several microRNAs related to the stem-like properties were discriminatively expressed in HCBP-1(+) subpopulation. Moreover, at least two distinct subpopulations in H460 tumor sphere cells could be distinguished by HCBP-1 peptide. Thus, a new method was established to identify lung CSCs/CSLCs, which provided robust approaches for the research of CSCs/CSLCs. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2014-12-01
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1623]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_朱毅敏团队
推荐引用方式 GB/T 7714	Zhu YM. Identification of stem-like cells in non-small cell lung cancer cells with specific peptides[J]. CANCER LETTERS,2014,351(1):100-107.
APA	Zhu YM.(2014).Identification of stem-like cells in non-small cell lung cancer cells with specific peptides.CANCER LETTERS,351(1),100-107.
MLA	Zhu YM."Identification of stem-like cells in non-small cell lung cancer cells with specific peptides".CANCER LETTERS 351.1(2014):100-107.