Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 马宏伟团队

	Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology
	Ma, HW(马宏伟)
刊名	BIOSENSORS & BIOELECTRONICS
	2013-03-15
卷号	41 期号:0 页码:710-716
关键词	Suspension array Microsphere/bead Residue Median fluorescent intensity (MFI)
通讯作者	Gao, ZX
英文摘要	Suspension array technology is proposed for the simultaneous quantitative determination of seven kinds of pesticide and veterinary drug residues, namely, atrazine, chloramphenicol, carbaryl, clenbuterol, 17-beta-estradiol, imidacloprid, and tylosin. The assay is simple and can be accomplished within 2 h without repeated pumping and washing steps unlike conventional suspension arrays. The hapten-protein conjugate-coated beads bind to their complementary biotinylated antibodies using a competitive immunoassay format. The coefficients of determination R-2 for six targets were greater than 0.992, whereas that for atrazine was 0.961, which indicate good logistic correlations. The dynamic ranges for the seven targets in the 7-plex assay ranged from 2 log units to 4 log units(1.60 x 10(0)-1.64 x 10(3), 5.12 x 10(-2)-1.60 x 10(2), 1.00 x 10(0)-3.13 x 10(3), 4.00 x 10(-1)-4.10 x 10(2), 4.00 x 10(-1)-4.10 x 10(2), 5.12 x 10(-2)-1.60 x 10(2), and 2.00 x 10(0)-4.00 x 10(2) ng mL(-1)). The minimum detection concentrations of chloramphenicol, carbaryl, clenbuterol and 17-beta-estradiol in the suspension array (0.05, 1.00, 0.40 and 0.40 ng mL(-1)) were lower than the corresponding limits of detection (0.25, 6.60, 24.23 and 13.96 ng mL(-1)) of using an indirect competitive enzyme-linked immunosorbent assay. Environmental scanning electron microscope was employed to characterize the bead surface, which directly confirmed the reactions on the beads. The suspension array is more flexible and feasible than ELISA for the fast quantitative analysis of pesticide and veterinary drug residues.
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000314191300106
公开日期	2014-01-15
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1411]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_马宏伟团队
推荐引用方式 GB/T 7714	Ma, HW. Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology[J]. BIOSENSORS & BIOELECTRONICS,2013,41(0):710-716.
APA	Ma, HW.(2013).Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology.BIOSENSORS & BIOELECTRONICS,41(0),710-716.
MLA	Ma, HW."Simultaneous and rapid detection of multiple pesticide and veterinary drug residues by suspension array technology".BIOSENSORS & BIOELECTRONICS 41.0(2013):710-716.