MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin. | |
Yi Cui(崔熠) ; Zhifeng Xiao ; Jin Han ; Jie Sun(孙杰) ; Wenyong Ding ; Yanhan Zhao ; Bing Chen ; Xiaoran Li(李晓然) ; Jianwu Dai* | |
刊名 | BMC Neurosci. |
2012-09-28 | |
卷号 | 13期号:13页码:116 |
关键词 | Neural stem/progenitor cells MiR-125b Nestin |
通讯作者 | Jianwu Dai* |
英文摘要 | BackgroundThe emerging concept is that microRNAs (miRNAs) play a central role in controlling stem cell self-renewal and fate determination by regulating the expression of stem cell regulators. miR-125b, one of neuronal miRNAs, recently was found to be necessary for neural differentiation of neural stem/progenitor cells (NS/PCs). However, the other specific biological role of miR-125b in NS/PCs is little known. We used rat NS/PCs as a model system to study the role of miR-125b in governing the behavior of NS/PCs. ResultsWe report here the transfection of exogenous miR-125b inhibited proliferation of NS/PCs but promoted differentiation and migration. Whereas anti-miR-125b had the opposite effect. Similar results were observed when Nestin was knocked down by siRNA. Subsequently, we demonstrated that Nestin was a direct functional target of miR-125b. MiR-125b downregulates the expression of luciferase through Nestin 3’untranslated region (3’-UTR), and the regulation was abolished by mutations in the miR-125b binding site. MiR-125b targeted the 3'-UTR of Nestin and reduced the abundance of Nestin at both mRNA and protein levels. ConclusionThe results provided new insight into the function by which miR-125b modulates NS/PCs proliferation, differentiation and migration. The data also indicated the regulatory role of miR-125b in NS/PCs might through the suppression of Nestin expression. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2013-01-22 |
内容类型 | 期刊论文 |
源URL | [http://58.210.77.100/handle/332007/980] |
专题 | 苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队 |
推荐引用方式 GB/T 7714 | Yi Cui,Zhifeng Xiao,Jin Han,et al. MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin.[J]. BMC Neurosci.,2012,13(13):116. |
APA | Yi Cui.,Zhifeng Xiao.,Jin Han.,Jie Sun.,Wenyong Ding.,...&Jianwu Dai*.(2012).MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin..BMC Neurosci.,13(13),116. |
MLA | Yi Cui,et al."MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin.".BMC Neurosci. 13.13(2012):116. |
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