The Effects of Topographical Patterns and Sizes on Neural Stem Cell Behavior

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 程国胜团队

	The Effects of Topographical Patterns and Sizes on Neural Stem Cell Behavior
	Kong, T（孔涛）; Su, RG（苏瑞巩）; Song, Q（宋琴）; Cheng, GS（程国胜）
刊名	PLOS ONE
	2013-03
卷号	8 期号:3
关键词	PROGENITOR CELLS NERVOUS-SYSTEM INDUCTION DIAMETER ADHESION DIFFERENTIATION GROWTH NANOTOPOGRAPHY MATRIX
通讯作者	Tang, ML（唐明亮）
英文摘要	Engineered topographical manipulation, a paralleling approach with conventional biochemical cues, has recently attracted the growing interests in utilizations to control stem cell fate. In this study, effects of topological parameters, pattern and size are emphasized on the proliferation and differentiation of adult neural stem cells (ANSCs). We fabricate micro-scale topographical Si wafers with two different feature sizes. These topographical patterns present linear micro-pattern (LMP), circular micro-pattern (CMP) and dot micro-pattern (DMP). The results show that the three topography substrates are suitable for ANSC growth, while they all depress ANSC proliferation when compared to non-patterned substrates (control). Meanwhile, LMP and CMP with two feature sizes can both significantly enhance ANSC differentiation to neurons compared to control. The smaller the feature size is, the better upregulation applies to ANSC for the differentiated neurons. The underlying mechanisms of topography-enhanced neuronal differentiation are further revealed by directing suppression of mitogen-activated protein kinase/extracellular signaling-regulated kinase (MAPK/Erk) signaling pathway in ANSC using U0126, known to inhibit the activation of Erk. The statistical results suggest MAPK/Erk pathway is partially involved in topography-induced differentiation. These observations provide a better understanding on the different roles of topographical cues on stem cell behavior, especially on the selective differentiation, and facilitate to advance the field of stem cell therapy.
收录类别	SCI
语种	英语
WOS记录号	WOS:000316411600028
公开日期	2014-01-15
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/1373]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_程国胜团队
推荐引用方式 GB/T 7714	Kong, T,Su, RG,Song, Q,et al. The Effects of Topographical Patterns and Sizes on Neural Stem Cell Behavior[J]. PLOS ONE,2013,8(3).
APA	Kong, T,Su, RG,Song, Q,&Cheng, GS.(2013).The Effects of Topographical Patterns and Sizes on Neural Stem Cell Behavior.PLOS ONE,8(3).
MLA	Kong, T,et al."The Effects of Topographical Patterns and Sizes on Neural Stem Cell Behavior".PLOS ONE 8.3(2013).