Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics

	Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics
	Gao YX(高愈希); Peng XM(彭晓敏); Zhao JT(赵甲亭); Gao, YX; Peng, XM; Zhang, JC; Zhao, JT; Li, YY; Li, YF; Li, B
刊名	METALLOMICS
	2013
卷号	5 期号:7 页码:913-919
英文摘要	The response of E. coli to Hg2+ exposure was investigated using proteomic and metalloproteomic approaches. E. coli was cultured in the LB medium containing HgCl2 and/or selenomethionine. The growth curve of E. coli was measured to estimate the toxicity of Hg2+ or selenomethionine. After two-dimensional gel electrophoresis (2-DE), distribution of Hg in 2-DE gel was detected with synchrotron radiation X-ray fluorescence (SRXRF) at 4W1B, Beijing Synchrotron Radiation Facility. The proteins with differential expression and those containing Hg were identified with electrospray ionization tandem mass spectrometry (ESI-MS/MS) and peptide mass fingerprinting analysis. The results showed that Hg2+ can inhibit the growth of E. coli, while supplement of selenomethionine can shorten the lag period induced by Hg2+, indicating an antagonistic effect of selenomethionine against Hg2+ toxicity. Mechanistically, Hg was observed to be able to bind pyruvate kinase, a glycolytic enzyme, and modulate the expression of five other proteins, including down-regulation of outer membrane protein W and up-regulation of transcription termination factor rho, cysteine synthase, transaldolase A and alkyl hydroperoxide reductase subunit C. Therefore, our results indicated that mercury may influence osmosis of plasma membrane, antioxidant defense, and glycometabolism of the microorganism. This study demonstrates the high sensitivity of SRXRF in identifying metal-associated proteins compared to conventional proteomic approaches.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
WOS记录号	WOS:000320951600018
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/224082]
专题	高能物理研究所_院士高能物理研究所_实验物理中心高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Gao YX,Peng XM,Zhao JT,et al. Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics[J]. METALLOMICS,2013,5(7):913-919.
APA	高愈希.,彭晓敏.,赵甲亭.,Gao, YX.,Peng, XM.,...&柴之芳.(2013).Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics.METALLOMICS,5(7),913-919.
MLA	高愈希,et al."Cellular response of E. coli upon Hg2+ exposure - a case study of advanced nuclear analytical approach to metalloproteomics".METALLOMICS 5.7(2013):913-919.