An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells

	An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells
	Zhang, HT; Xie, YS; Wang, P; Chen, GC; Liu, RC; Lam, YW; Hu, Y; Zhu, Q; Sun, HY; Hu Y(胡毅)
刊名	TALANTA
	2015
卷号	135 页码:149-154
关键词	Fluorescent probe Hydrogen sulfide Cell imaging Iminocoumarin benzothiazole
英文摘要	Hydrogen sulfide (H2S) has recently been identified as the third gaseous signaling molecule that is involved in regulating many important cellular processes. We report herein a novel fluorescent probe for detecting H2S based on iminocoumarin benzothiazole scaffold. The probe displayed high sensitivity and around 80-fold increment in fluorescence signal after reacting with H2S under physiological condition. The fluorescent intensity of the probe was linearly related to H2S concentration in the range of 0-100 mu M with a detection limit of 0.15 mu M (3 sigma/slope). The probe also showed excellent selectivity towards H2S over other biologically relevant species, including ROS, RSS and RNS. Its selectivity for H2S is 32 folds higher than other reactive sulfur species. Furthermore, the probe has been applied for imaging H2S in living cells. Cell imaging experiments demonstrated that the probe is cell-permeable and can be used to monitor the alteration of H2S concentrations in living cells. We envisage that this probe can provide useful tools to further elucidate the biological roles of H2S. (C) 2015 Elsevier B.V. All rights reserved.
学科主题	Chemistry
类目[WOS]	Chemistry, Analytical
收录类别	SCI ; EI
WOS记录号	WOS:000349730700022
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/228072]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Zhang, HT,Xie, YS,Wang, P,et al. An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells[J]. TALANTA,2015,135:149-154.
APA	Zhang, HT.,Xie, YS.,Wang, P.,Chen, GC.,Liu, RC.,...&胡毅.(2015).An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells.TALANTA,135,149-154.
MLA	Zhang, HT,et al."An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells".TALANTA 135(2015):149-154.