Study of Pb2+ on the mechanism of trypsin activity

	Study of Pb2+ on the mechanism of trypsin activity
	Hong, FS; Wang, L; Wu, K; Wang, XF; Liu, C; Tao, Y; Tao Y(陶冶)
刊名	CHINESE JOURNAL OF INORGANIC CHEMISTRY
	2003
卷号	19 期号:2 页码:#REF!
关键词	trypsin lead ion enzyme activity EXAFS secondary structure
其他题名	Pb2+ 对胰蛋白酶活性影响的作用机理研究
通讯作者	Hong, FS (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
英文摘要	The activity of trypsin from bovine pancreas was enhanced under the treatment by Ph2+ at low concentration (0.5 similar to 3 mumol (.) L-1) but was inhibited by Pb2+ at high concentration (3 mumol (.) L-1 above). Pb2+ at high concentration could competitively displace Ca2+ from trypsin. The EXAFS demonstrated that Pb2+ bound to polypeptide chain of trypsin, coordination atom was nitrogen or oxygen, its coordination number was 2 and Pb-N (or 0) bond length was 0.241 nm. The secondary structure of trypsin was greatly changed by Pb2+ at high concentration, e. g. a-helix, beta-turn and Random coil contents decreased and beta-sheet increased. It suggested that Pb2+ bound result in trypsin conformational changed, and enzyme activity decreased.
学科主题	Chemistry
类目[WOS]	Chemistry, Inorganic & Nuclear
收录类别	SCI
WOS记录号	WOS:000180653400004
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/226365]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Hong, FS,Wang, L,Wu, K,et al. Study of Pb2+ on the mechanism of trypsin activity[J]. CHINESE JOURNAL OF INORGANIC CHEMISTRY,2003,19(2):#REF!.
APA	Hong, FS.,Wang, L.,Wu, K.,Wang, XF.,Liu, C.,...&陶冶.(2003).Study of Pb2+ on the mechanism of trypsin activity.CHINESE JOURNAL OF INORGANIC CHEMISTRY,19(2),#REF!.
MLA	Hong, FS,et al."Study of Pb2+ on the mechanism of trypsin activity".CHINESE JOURNAL OF INORGANIC CHEMISTRY 19.2(2003):#REF!.