Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach

	Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach
	Hong, FS; Wang, XF; Liu, C; Su, GX; Song, WP; Wu, K; Tao, Y; Zhao, GW; Tao Y(陶冶)
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2003
卷号	46 期号:1 页码:#REF!
关键词	Ce3+ spinach D1/D2/Cytb559 complex photosynthesis spectra characteristic
通讯作者	Hong, FS (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
英文摘要	It was studied by spectroscopy that PSII reaction center complex consisting of three polypeptides, D-1, D-2 and Cytb559, were purified from PSII particle of CeCl3 treated spinach. The results of the experiment show that Ce3+, could improve the growth of spinach, and accelerate electron transport of PSII particles. Of chl-a. of UIV-Vis spectrum of D1/D2/Cytb559 complex, Soret band was blue-shifted by 3 nm and Q band by 2 nm, respectively, and the fluorescence emission peak was blue-shifted by 5 nm in CeCl3-treated spinach compared with the one in control. By the extended X-ray absorption fine structure (EXAFS) spectroscopy methods, it has been found that Ce3+ is coordinated with 8 nitrogen atoms in the first coordination shell with Ce-N bond length of 0.253 nm, and Ce3+ with 6 oxygen atoms in the second coordination shell with Ce-O bond length of 0.32 nm. However, the secondary structure of D1/D2/Cytb559 complex by circular dichroism (CD) spectroscopy has no significant change after CeCl3 treated. It might be that Ce3+ binds to porphyrin rings of chlorophyll and oxygen of amino acid residue of polypeptide in D1/D2/Cytb559 complex, and then accelerates the primary reaction of PSII, intensifies function of P680(+) primary electron donor of Dl/D2/Cytb559, but there is little change in conformation of PSII reaction center complex.
学科主题	Chemistry
类目[WOS]	Chemistry, Multidisciplinary
收录类别	SCI
WOS记录号	WOS:000181415100007
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/225695]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Hong, FS,Wang, XF,Liu, C,et al. Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2003,46(1):#REF!.
APA	Hong, FS.,Wang, XF.,Liu, C.,Su, GX.,Song, WP.,...&陶冶.(2003).Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach.SCIENCE IN CHINA SERIES B-CHEMISTRY,46(1),#REF!.
MLA	Hong, FS,et al."Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach".SCIENCE IN CHINA SERIES B-CHEMISTRY 46.1(2003):#REF!.