Size-dependent impact of CNTs on dynamic properties of calmodulin

	Size-dependent impact of CNTs on dynamic properties of calmodulin
	Gao J(高剑); Gao, J; Wang, LM; Kang, SG; Zhao, LN; Ji, MJ; Chen, CY; Zhao, YL; Zhou, RH; Li, JY;
刊名	NANOSCALE
	2014
卷号	6 期号:21 页码:12828-12837
英文摘要	There are growing concerns about the biosafety of nanomaterials such as carbon nanotubes (CNTs) as their applications become more widespread. We report here a theoretical and experimental study of the binding of various sizes of CNTs [CNT (4,4), (5,5), (6,6) and (7,7)] to calmodulin (CaM) protein and, in particular, their impact on the Ca2+-dependent dynamic properties of CaM. Our simulations show that all the CNTs can plug into the hydrophobic binding pocket of Ca2+-bound CaM with binding affinities comparable with the native substrate M13 peptide. Even though CNT (4,4) shows a similar behavior to the M13 peptide in its dissociation from Ca2+-free CaM, wider CNTs still bind firmly to CaM, indicating a potential failure of Ca2+ regulation. Such a size-dependent impact of CNTs on the dynamic properties of CaM is a result of the excessively strong hydrophobic interactions between the wider CNTs and CaM. These simulation results were confirmed by circular dichroism spectroscopy, which showed that the secondary structures of CaM become insensitive to Ca2+ concentrations after the addition of CNTs. Our findings indicate that the cytotoxicity of nanoparticles to proteins arises not only from the inhibition of static protein structures (binding pockets), but also from impacts on their dynamic properties.
学科主题	Chemistry; Science & Technology - Other Topics; Materials Science; Physics
收录类别	SCI
WOS记录号	WOS:000344997500076
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/225200]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Gao J,Gao, J,Wang, LM,et al. Size-dependent impact of CNTs on dynamic properties of calmodulin[J]. NANOSCALE,2014,6(21):12828-12837.
APA	高剑.,Gao, J.,Wang, LM.,Kang, SG.,Zhao, LN.,...&李敬源.(2014).Size-dependent impact of CNTs on dynamic properties of calmodulin.NANOSCALE,6(21),12828-12837.
MLA	高剑,et al."Size-dependent impact of CNTs on dynamic properties of calmodulin".NANOSCALE 6.21(2014):12828-12837.