Revealing the Binding Structure of the Protein Corona on Gold Nanorods Using Synchrotron Radiation-Based Techniques: Understanding the Reduced Damage in Cell Membranes

	Revealing the Binding Structure of the Protein Corona on Gold Nanorods Using Synchrotron Radiation-Based Techniques: Understanding the Reduced Damage in Cell Membranes
	Wang LM(王黎明); Li JY(李敬源); Pan J(潘俊); Wang, LM; Li, JY; Pan, J; Jiang, XM; Ji, YL; Li, YF; Qu, Y
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2013
卷号	135 期号:46 页码:17359-17368
英文摘要	Regarding the importance of the biological effects of nanomaterials, there is still limited knowledge about the binding structure and stability of the protein corona on nanomaterials and the subsequent impacts. Here we designed a hard serum albumin protein corona (BSA) on CTAB-coated gold nanorods (AuNRs) and captured the structure of protein adsorption using synchrotron radiation X-ray absorption spectroscopy, microbeam X-ray fluorescent spectroscopy, and circular dichroism in combination with molecular dynamics simulations. The protein adsorption is attributed to at least 12 Au-S bonds and the stable corona reduced the cytotoxicity of CTAB/AuNRs. These combined strategies using physical, chemical, and biological approaches will improve our understanding of the protective effects of protein coronas against the toxicity of nanomaterials. These findings have shed light on a new strategy for studying interactions between proteins and nanomaterials, and this information will help further guide the rational design of nanomaterials for safe and effective biomedical applications.
学科主题	Chemistry
收录类别	SCI
WOS记录号	WOS:000327413300025
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/224355]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Wang LM,Li JY,Pan J,et al. Revealing the Binding Structure of the Protein Corona on Gold Nanorods Using Synchrotron Radiation-Based Techniques: Understanding the Reduced Damage in Cell Membranes[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2013,135(46):17359-17368.
APA	王黎明.,李敬源.,潘俊.,Wang, LM.,Li, JY.,...&陈春英.(2013).Revealing the Binding Structure of the Protein Corona on Gold Nanorods Using Synchrotron Radiation-Based Techniques: Understanding the Reduced Damage in Cell Membranes.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,135(46),17359-17368.
MLA	王黎明,et al."Revealing the Binding Structure of the Protein Corona on Gold Nanorods Using Synchrotron Radiation-Based Techniques: Understanding the Reduced Damage in Cell Membranes".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 135.46(2013):17359-17368.