Separation and purification of fullerenols for improved biocompatibility

	Separation and purification of fullerenols for improved biocompatibility
	Li J(李娟); Zhang MY(张铭倚); Sun BY(孙宝云); Li, J; Zhang, MY; Sun, BY; Xing, GM; Song, Y; Guo, HL; Chang, YA
刊名	CARBON
	2012
卷号	50 期号:2 页码:460-469
英文摘要	Isoelectric focusing was used to separate the different components of fullerenols based on their different electronegativities induced by the surface chemical functionalities and structures. Purified fractions of fullerenols were analyzed and characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that four fractions of fullerenols could be separated by isoelectric focusing, and all fractions had a general formula of C-60(OH)(m)(O)(n) where the values of m and n might vary with their isoelectric points. Results from the capillary electrophoresis studies showed that the negative surface charge densities were different among the fractions of fullerenols, which indicated that the net surface negative charge was determined by the degree of C=O functionalization. In vitro cytotoxicity assays were also performed to determine the effect of purified fullerenols on cytotoxicity and DNA stability in Raw 264.7 cells. Although the fullerenols had low cytotoxicity, the composition of fullerenols with the highest negative charge density (-1.913 +/- 0.008 x 10(-4) C) and smaller size (3.92 +/- 0.71 nm) was found to induce DNA damage. Therefore, crude fullerenols must be separated and purified in order to obtain fullerenols with low degree of C=O functionalization and good biocompatibility. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Chemistry; Materials Science
收录类别	SCI
WOS记录号	WOS:000297397700014
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/223988]
专题	高能物理研究所_多学科研究中心
推荐引用方式 GB/T 7714	Li J,Zhang MY,Sun BY,et al. Separation and purification of fullerenols for improved biocompatibility[J]. CARBON,2012,50(2):460-469.
APA	李娟.,张铭倚.,孙宝云.,Li, J.,Zhang, MY.,...&常亚男.(2012).Separation and purification of fullerenols for improved biocompatibility.CARBON,50(2),460-469.
MLA	李娟,et al."Separation and purification of fullerenols for improved biocompatibility".CARBON 50.2(2012):460-469.