Revealing the carbohydrate pattern on a cell surface by super-resolution imaging

	Revealing the carbohydrate pattern on a cell surface by super-resolution imaging
	Chen,Junling; Gao,Jing; Wu,Jiazhen; Zhang,Min; Cai,Mingjun; Xu,Haijiao; Jiang,Junguang; Tian,Zhiyuan; Wang,Hongda
刊名	nanoscale
	2015
卷号	7 期号:8 页码:3373-3380
关键词	OPTICAL RECONSTRUCTION MICROSCOPY FLUORESCENT-PROBES MEMBRANE GLYCANS LOCALIZATION RECOGNITION GLYCOMICS DISEASE STORM LIMIT
通讯作者	wang,hd
英文摘要	carbohydrates are involved in various physiological and pathological activities including cell adhesion, signal transduction and tumor invasion. the distribution of carbohydrates is the molecular basis of their multiple functions, but remains poorly understood. here, we employed direct stochastic optical reconstruction microscopy (dstorm) to visualize the pattern of n-acetylglucosamine (n-glcnac) on vero cell membranes at the nanometer level of resolution. we found that n-glcnacs exist in irregular clusters on the apical membrane with an average cluster area of about 0.37 mu m(2). most of these n-glcnac clusters are co-localized with lipid rafts by dual-color dstorm imaging, suggesting that carbohydrates are closely associated with lipid rafts as the functional domains. our results demonstrate that super-resolution imaging is capable of characterizing the distribution of carbohydrates on the cellular surface at the molecular level.
收录类别	SCI
语种	英语
公开日期	2016-05-03
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64662]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Chen,Junling,Gao,Jing,Wu,Jiazhen,et al. Revealing the carbohydrate pattern on a cell surface by super-resolution imaging[J]. nanoscale,2015,7(8):3373-3380.
APA	Chen,Junling.,Gao,Jing.,Wu,Jiazhen.,Zhang,Min.,Cai,Mingjun.,...&Wang,Hongda.(2015).Revealing the carbohydrate pattern on a cell surface by super-resolution imaging.nanoscale,7(8),3373-3380.
MLA	Chen,Junling,et al."Revealing the carbohydrate pattern on a cell surface by super-resolution imaging".nanoscale 7.8(2015):3373-3380.