Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform

	Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform
	Wang,Zhenzhen; Chen,Zhaowei; Gao,Nan; Ren,Jinsong; Qu,Xiaogang
刊名	small
	2015
卷号	11 期号:37 页码:4970-4975
关键词	POINT-OF-CARE QUANTITATIVE DETECTION BACTERIAL DETECTION GLUCOMETER READOUT RAPID DETECTION DNA IDENTIFICATION NANOPARTICLE SYSTEMS PROBES
通讯作者	ren,js
英文摘要	herein, for the first time, we presented a simple and general approach by using personal glucose meters (pgm) for portable and ultrasensitive detection of microbial pathogens. upon addition of pathogenic bacteria, glucoamylase-quaternized magnetic nanoparticles (ga-qmnps) conjugates were disrupted by the competitive multivalent interactions between bacteria and qmnps, resulting in the release of ga. after magnetic separation, the free ga could catalyze the hydrolysis of amylose into glucose for quantitative readout by pgm. in such way, pgm was transmuted into a bacterial detection device and extremely low detection limits down to 20 cells ml(-1) was achieved. more importantly, qmnps could inhibit the growth of the bacteria and destroy its cellular structure, which enabled bacteria detection and inhibition simultaneously. the simplicity, portability, sensitivity and low cost of presented work make it attractive for clinical applications.
收录类别	SCI
语种	英语
WOS记录号	WOS:000362819400020
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64600]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang,Zhenzhen,Chen,Zhaowei,Gao,Nan,et al. Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform[J]. small,2015,11(37):4970-4975.
APA	Wang,Zhenzhen,Chen,Zhaowei,Gao,Nan,Ren,Jinsong,&Qu,Xiaogang.(2015).Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform.small,11(37),4970-4975.
MLA	Wang,Zhenzhen,et al."Transmutation of Personal Glucose Meters into Portable and Highly Sensitive Microbial Pathogen Detection Platform".small 11.37(2015):4970-4975.