Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane

	Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
	Pan,Yangang; Wang,Shaowen; Shan,Yuping; Zhang,Dinglin; Gao,Jing; Zhang,Min; Liu,Shuheng; Cai,Mingjun; Xu,Haijiao; Li,Guohui
刊名	small
	2015
卷号	11 期号:23 页码:2782-2788
关键词	SINGAPORE GROUPER IRIDOVIRUS INDIVIDUAL INFLUENZA-VIRUSES ATOMIC-FORCE MICROSCOPY HOST-CELLS PROTEOMIC ANALYSIS SPOTTED GROUPER ENTRY ENDOCYTOSIS MACROPINOCYTOSIS SPECTROSCOPY
通讯作者	li,gh
英文摘要	the first step in most viral infections is the penetration of the cell membrane via endocytosis. however, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. here, the endocytosis of a single live virion (singapore grouper iridovirus, sgiv) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. for the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single sgiv by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pn, and the binding energy density increases with the engulfment depth. this report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level.
收录类别	SCI
语种	英语
WOS记录号	WOS:000356448300011
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/64595]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Pan,Yangang,Wang,Shaowen,Shan,Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. small,2015,11(23):2782-2788.
APA	Pan,Yangang.,Wang,Shaowen.,Shan,Yuping.,Zhang,Dinglin.,Gao,Jing.,...&Li,Guohui.(2015).Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane.small,11(23),2782-2788.
MLA	Pan,Yangang,et al."Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane".small 11.23(2015):2782-2788.