Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin
	Wang, XY ; Yu, JL ; Kang, Q ; Shen, DZ ; Li, JH; Chen, LX
刊名	BIOSENSORS & BIOELECTRONICS
	2016-03-15
卷号	77 页码:624-630
关键词	Molecular imprinting Ratiometric fluorescence Fluorescence resonance energy transfer Phycocyanin
ISSN号	0956-5663
通讯作者	Shen, DZ (reprint author), Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China. dzshen@sdnu.edu.cn ; lxchen@yic.ac.cn
产权排序	[Wang, Xiaoyan; Yu, Jialuo; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ,Shandong Pr, Jinan 250014, Peoples R China; [Wang, Xiaoyan; Yu, Jialuo; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
英文摘要	A facile strategy was developed to prepare molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin (PC) based on fluorescence resonance energy transfer (FRET), via a sal-gel polymerization process using nitrobenzoxadiazole (NBD) as fluorescent signal source. The ratio of two fluorescence peak emission intensities of NBD and PC was utilized to determine the concentration of PC, which could effectively reduce the background interference and fluctuation of diverse conditions. As a result, this sensor obtained high sensitivity with a low detection limit of 0.14 nM within 6 min, and excellent recognition specificity for PC over its analogues with a high imprinting factor of 9.1. Furthermore, the sensor attained high recoveries in the range of 93.8-110.2% at three spiking levels of PC, with precisions below 4.7% in seawater and lake water samples. The developed sensor strategy demonstrated simplicity, reliability, rapidity, high selectivity and high sensitivity, proving to be a feasible way to develop high efficient fluorescence sensors and thus potentially applicable for ultratrace analysis of complicated matrices. (C) 2015 Elsevier B.V. All rights reserved.
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	QUANTUM DOTS ; IONS ; NANOPARTICLES ; POLYMER ; MONOMER ; HYBRID ; PROBE ; SITE ; TNT
收录类别	SCI
语种	英语
WOS记录号	WOS:000366766900090
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9314]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes,Minist Educ 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 3.Binzhou Med Univ, Sch Pharm
推荐引用方式 GB/T 7714	Wang, XY,Yu, JL,Kang, Q,et al. Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin[J]. BIOSENSORS & BIOELECTRONICS,2016,77:624-630.
APA	Wang, XY,Yu, JL,Kang, Q,Shen, DZ,Li, JH,&Chen, LX.(2016).Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin.BIOSENSORS & BIOELECTRONICS,77,624-630.
MLA	Wang, XY,et al."Molecular imprinting ratiometric fluorescence sensor for highly selective and sensitive detection of phycocyanin".BIOSENSORS & BIOELECTRONICS 77(2016):624-630.