A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action
	Zhang, LB; Yang, DL; Wang, Q; Yuan, ZY; Wu, HF; Pei, D; Cong, M; Li, F; Ji, CL; Zhao, JM
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2015-07-01
卷号	51 期号:1 页码:29-38
关键词	Venerupis philippinarum Defensin Molecular characterization Tissue distribution Antimicrobial activity Membrane lesions
ISSN号	0145-305X
通讯作者	Wu, HF (reprint author), YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. hfwu@yic.ac.cn ; jmzhao@yic.ac.cn
产权排序	[Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Peoples R China; [Zhang, Linbao; Yang, Dinglong; Wang, Qing; Wu, Huifeng; Pei, Dong; Cong, Ming; Li, Fei; Ji, Chenglong; Zhao, Jianmin] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yuan, Zeyi] Natl Marine Data & Informat Serv, Tianjin 300171, Peoples R China
英文摘要	Antimicrobial peptides (AMPs) are important mediators of the primary host defense system against microbial invasion. In the present study, we cloned and characterized a member of the invertebrate defensin from the clam Venerupis philippinarum, designated VpDef. Amino acid sequence analysis showed that VpDef was similar to defensins from marine mollusks and ticks. In non-stimulated clams, RT-PCR and immunohistochemical analysis revealed that both VpDef mRNA and the encoding peptide were constitutively expressed in hemocytes and mantles, as well as in other major tissues. VpDef transcripts were significantly induced in hemocytes at different time intervals post Vibrio anguillarum infection. The recombinant VpDef (rVpDef) showed the highest activity against Gram-positive bacteria Micrococcus luteus and less effective to Gram-negative bacteria. In addition, incubation of rVpDef with M. luteus at 1 x and 3 x MIC could induce an obvious decrease of the membrane potential and notable changes of membrane permeability in a dose-dependent manner. Membrane integrity and bacterial viability analysis also revealed that rVpDef increased the membrane permeability of M. luteus and then resulted in cell death at 2 x and 10 x MIC. Overall, these results suggest that VpDef has an important function in host defense against invasive pathogens, probably killing microbes by inducing membrane lesions. (C) 2015 Elsevier Ltd. All rights reserved.
研究领域[WOS]	Immunology ; Zoology
关键词[WOS]	OYSTER CRASSOSTREA-GIGAS ; RICH ANTIMICROBIAL PEPTIDES ; PRECURSOR LIPID II ; MARINE-INVERTEBRATES ; ESCHERICHIA-COLI ; RUDITAPES-PHILIPPINARUM ; MICROCOCCUS-LUTEUS ; ARTHROPOD DEFENSIN ; INNATE IMMUNITY ; MEMBRANE
收录类别	SCI
语种	英语
WOS记录号	WOS:000357906800004
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9274]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室烟台海岸带研究所_污染过程与控制实验室海岸带生物资源高效利用研究与发展中心
推荐引用方式 GB/T 7714	Zhang, LB,Yang, DL,Wang, Q,et al. A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2015,51(1):29-38.
APA	Zhang, LB.,Yang, DL.,Wang, Q.,Yuan, ZY.,Wu, HF.,...&Zhao, JM.(2015).A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,51(1),29-38.
MLA	Zhang, LB,et al."A defensin from clam Venerupis philippinarum: Molecular characterization, localization, antibacterial activity, and mechanism of action".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 51.1(2015):29-38.