A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo
	Yu, FB; Gao, M; Li, M; Chen, LX
刊名	BIOMATERIALS
	2015-09-01
卷号	63 页码:93-101
关键词	Fluorescent probe Image analysis Hydrogen polysulfides Superoxide anion Near-infrared
ISSN号	0142-9612
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
产权排序	[Yu, Fabiao; Gao, Min; Li, Meng; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
英文摘要	Intracellular reactive sulfur species play important roles in physiological and pathological processes. Emerging evidences suggest that sulfane sulfur instead of H2S is the actual signaling molecule in these processes. Sulfane sulfur can be generated as a result of the reaction between O-2 and H2S in mitochondria. Therefore, we develop a near-infrared mitochondria-targeting probe that allows multiresponse to O-2 and H2SO successively for investigating this biosynthetic reaction. The probe exhibits highly selective fluorescent response to O-2 and H2SO successively in presence of potential biological interferants. Fluorescent imaging studies and flow cytometry analysis of RAW264.7 cells elaborate that the probe can be used to reveal the continuous process of O-2 burst and H2SO production in situ and in real-time. The mitochondria isolation indicates that the probe can specifically localize in mitochondria. Finally, the fluorescent probe has been successfully applied to detect O-2 and H2Sn in mice. (C) 2015 Elsevier Ltd. All rights reserved.
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	DIFFERENT EMISSION CHANNELS ; REACTIVE OXYGEN ; LIVING CELLS ; OXIDATIVE STRESS ; LIVE CELLS ; HYPOCHLOROUS ACID ; SULFANE SULFUR ; SULFIDE ; PEROXYNITRITE ; H2S
收录类别	SCI
语种	英语
WOS记录号	WOS:000357910400009
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/9271]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
推荐引用方式 GB/T 7714	Yu, FB,Gao, M,Li, M,et al. A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo[J]. BIOMATERIALS,2015,63:93-101.
APA	Yu, FB,Gao, M,Li, M,&Chen, LX.(2015).A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo.BIOMATERIALS,63,93-101.
MLA	Yu, FB,et al."A dual response near-infrared fluorescent probe for hydrogen polysulfides and superoxide anion detection in cells and in vivo".BIOMATERIALS 63(2015):93-101.