Study on the interaction of a cyanine dye with human serum transferrin

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	Study on the interaction of a cyanine dye with human serum transferrin
	Zhang, Xiu-feng 1; Chen, Lei 1; Yang, Qian-fan 2; Li, Qian 2; Sun, Xiao-ran 1; Chen, Hong-bo 1; Yang, Guang 1; Tang, Ya-lin 2
刊名	LUMINESCENCE
	2015-12-01
卷号	30 期号:8 页码:1176-1183
关键词	transferrin cyanine dye fluorescent quenching conformational transition docking
英文摘要	Complexation between the primary carrier of ligands in blood plasma, human serum transferrin (Tf), and a cyanine dye, 3,3'-di(3-sulfopropyl)-4,5,4', 5'-dibenzo-9-phenyl-thiacarbocyanine-triethylam monium salt (PTC) was investigated using fluorescence spectra, UV/Vis absorption spectra, synchronous fluorescence spectra, circular dichroism (CD) and molecular dynamic docking. The experimental results demonstrate that the formation of PTC-Tf complex is stabilized by van der Waal's interactions and hydrogen bonds, and the binding constants were found to be 8.55 x 10(6), 8.19 x 10(6) and 1.75 x 10(4) M-1. Moreover, fluorescence experiments prove that the operational mechanism for the fluorescence quenching is static quenching and non-radiative energy transfer. Structural investigation of the PTC-Tf complexes via synchronous fluorescence spectra and CD showed that the structure of Tf became more stable with a major increase in the a-helix content and increased polarity around the tryptophan residues after PTC binding. In addition, molecular modeling highlights the residues located in the N-lobe, which retain high affinity for PTC. The mode of action of the PTC-Tf complex is illustrated by these results, and may provide an effective pathway for the transport and targeted delivery of antitumor agents. Copyright (C) 2015 John Wiley & Sons, Ltd.
收录类别	SCI
语种	英语
公开日期	2016-03-15
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/29756]
专题	化学研究所_分子动态与稳态结构实验室
作者单位	1.Hebei United Univ, Coll Chem Engn, Tangshan 063009, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiu-feng,Chen, Lei,Yang, Qian-fan,et al. Study on the interaction of a cyanine dye with human serum transferrin[J]. LUMINESCENCE,2015,30(8):1176-1183.
APA	Zhang, Xiu-feng.,Chen, Lei.,Yang, Qian-fan.,Li, Qian.,Sun, Xiao-ran.,...&Tang, Ya-lin.(2015).Study on the interaction of a cyanine dye with human serum transferrin.LUMINESCENCE,30(8),1176-1183.
MLA	Zhang, Xiu-feng,et al."Study on the interaction of a cyanine dye with human serum transferrin".LUMINESCENCE 30.8(2015):1176-1183.