Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus
	Wen, Aiyun 1,2; You, Feng 1; Sun, Peng 1; Li, Jun 1; Xu, Dongdong 1; Wu, Zhihao 1; Ma, Deyou 1; Zou, Yuxia 1; Tan, Xungang1 ; Fan, Zhaofei1
刊名	ANIMAL BIOLOGY
	2015
卷号	65 期号:3-4 页码:193-207
关键词	Expression patterns gonadal differentiation Paralichthys olivaceus sex related genes
英文摘要	The present study aims to elucidate the different expression patterns and possible roles of Doublesex and Mab-3-related transcription factor 1 (dmrt1), dmrt4, SRY-related transcription factor 9 (sox9) and cytochrome P450 aromatase 19a (cyp19a) during gonadal differentiation in olive flounder, Paralichthys olivaceus. We first analyzed the gene expression patterns in tissues using RT-PCR, which indicated dmrt1, sox9 and cyp19a were sex-related genes with sexual dimorphic expression. The quantitative expression changes of these three genes together with dmrt4 during gonadal differentiation were further examined using real-time RT-PCR. The results showed that dmrt1 was scarcely expressed in the primitive gonad and during following periods of gonadal differentiation. Its expression increased rapidly in the differentiating testis. Dmrt4 was strongly expressed in primitive gonads and much less expressed during following periods of gonadal differentiation. Its expression became strong in differentiating testes. While sox9 was highly expressed in the primitive gonad, it was expressed with fluctuations during following periods of gonadal differentiation. Cyp19a started expressing in primitive gonads, and its expression quantity fluctuated during latter periods of gonadal differentiation, but was strongly expressed in the early stage of differentiating ovaries. Results of in situ hybridization showed that dmrt4 and sox9 transcripts were both mainly localized in spermatocytes and our results suggested these four sex-related genes might be involved in gonadal differentiation through their synergistic effects in flounder.
收录类别	SCI
语种	英语
WOS记录号	WOS:000367519100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/95208]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wen, Aiyun,You, Feng,Sun, Peng,et al. Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus[J]. ANIMAL BIOLOGY,2015,65(3-4):193-207.
APA	Wen, Aiyun.,You, Feng.,Sun, Peng.,Li, Jun.,Xu, Dongdong.,...&Zhang, Peijun.(2015).Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus.ANIMAL BIOLOGY,65(3-4),193-207.
MLA	Wen, Aiyun,et al."Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus".ANIMAL BIOLOGY 65.3-4(2015):193-207.