Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo

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	Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo
	Liu, YG; Miao, QL; Yuan, JC; Han, SE; Zhang, PP; Li, SL; Rao, ZP; Zhao, WL; Ye, Q; Geng, JL
刊名	JOURNAL OF NEUROSCIENCE
	2015
卷号	35 期号:25 页码:9336-9355
关键词	CENTRAL-NERVOUS-SYSTEM NEURAL STEM-CELLS ADULT SPINAL-CORD DOPAMINERGIC-NEURONS HUMAN FIBROBLASTS FLUORESCENT PROTEIN GABAERGIC NEURONS PRONEURAL GENES BRAIN DIFFERENTIATION
通讯作者	Cheng, LP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,xhzhang@bnu.edu.cn ; lpcheng@ion.ac.cn
英文摘要	In vivo induction of non-neuronal cells into neurons by transcription factors offers potential therapeutic approaches for neural regeneration. Although generation of induced neuronal (iN) cells in vitro and in vivo has been reported, whether iN cells can be fully integrated into existing circuits remains unclear. Here we show that expression of achaete-scute complex homolog-like 1 (Ascl1) alone is sufficient to convert dorsal midbrain astrocytes of mice into functional iN cells in vitro and in vivo. Specific expression of Ascl1 in astrocytes by infection with GFAP-adeno-associated virus (AAV) vector converts astrocytes in dorsal midbrain, striatum, and somatosensory cortex of postnatal and adult mice into functional neurons in vivo. These iN cells mature progressively, exhibiting neuronal morphology and markers, action potentials, and synaptic inputs from and output to existing neurons. Thus, a single transcription factor, Ascl1, is sufficient to convert brain astrocytes into functional neurons, and GFAP-AAVis an efficient vector for generating iN cells from astrocytes in vivo.
学科主题	Neurosciences
WOS标题词	Neurosciences & Neurology
收录类别	SCI
语种	英语
WOS记录号	WOS:000358252000010
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/3942]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Liu, YG,Miao, QL,Yuan, JC,et al. Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo[J]. JOURNAL OF NEUROSCIENCE,2015,35(25):9336-9355.
APA	Liu, YG.,Miao, QL.,Yuan, JC.,Han, SE.,Zhang, PP.,...&Cheng, LP.(2015).Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo.JOURNAL OF NEUROSCIENCE,35(25),9336-9355.
MLA	Liu, YG,et al."Ascl1 Converts Dorsal Midbrain Astrocytes into Functional Neurons In Vivo".JOURNAL OF NEUROSCIENCE 35.25(2015):9336-9355.