Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin

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	Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin
	Wong, YH; Lee, CM; Xie, WJ; Cui, BX; Poo, MM
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2015
卷号	112 期号:32 页码:E4475-E4484
关键词	LONG-TERM POTENTIATION NEUROTROPHIC FACTOR SYNAPTIC VESICLE NEUROTRANSMITTER RELEASE TRANSMITTER SECRETION AXONAL-TRANSPORT QUANTUM DOTS NEURONS LTP EXOCYTOSIS
通讯作者	Poo, MM (reprint author), Univ Calif Berkeley, Dept Mol & Cell Biol, Div Neurobiol, Helen Wills Neurosci Inst, 229 Stanley Hall, Berkeley, CA 94720 USA.,mpoo@berkeley.edu
英文摘要	Brain-derived neurotrophic factor (BDNF) is known to modulate synapse development and plasticity, but the source of synaptic BDNF and molecular mechanisms regulating BDNF release remain unclear. Using exogenous BDNF tagged with quantum dots (BDNF-QDs), we found that endocytosed BDNF-QDs were preferentially localized to postsynaptic sites in the dendrite of cultured hippocampal neurons. Repetitive neuronal spiking induced the release of BDNF-QDs at these sites, and this process required activation of glutamate receptors. Down-regulating complexin 1/2 (Cpx1/2) expression eliminated activity-induced BDNF-QD secretion, although the overall activity-independent secretion was elevated. Among eight synaptotagmin (Syt) isoforms examined, down-regulation of only Syt6 impaired activity-induced BDNF-QD secretion. In contrast, activity-induced release of endogenously synthesized BDNF did not depend on Syt6. Thus, neuronal activity could trigger the release of endosomal BDNF from postsynaptic dendrites in a Cpx- and Syt6-dependent manner, and endosomes containing BDNF may serve as a source of BDNF for activity-dependent synaptic modulation.
学科主题	Multidisciplinary Sciences
WOS标题词	Science & Technology - Other Topics
收录类别	SCI
语种	英语
WOS记录号	WOS:000359285100019
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/3936]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wong, YH,Lee, CM,Xie, WJ,et al. Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2015,112(32):E4475-E4484.
APA	Wong, YH,Lee, CM,Xie, WJ,Cui, BX,&Poo, MM.(2015).Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,112(32),E4475-E4484.
MLA	Wong, YH,et al."Activity-dependent BDNF release via endocytic pathways is regulated by synaptotagmin-6 and complexin".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 112.32(2015):E4475-E4484.