Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails
	Fu, YB; Huang, CW; Xu, XX; Gu, HF; Ye, YQ; Jiang, CZ; Qiu, ZL; Xie, X
刊名	CELL RESEARCH
	2015
卷号	25 期号:9 页码:1013-1024
关键词	PLURIPOTENT STEM-CELLS ISCHEMIC-HEART-DISEASE IN-VIVO CARDIAC FIBROBLASTS PROGENITOR CELLS SOMATIC-CELLS ADULT HEART TGF-BETA DIFFERENTIATION REPAIR
通讯作者	Xie, X (reprint author), Tongji Univ, Shanghai Key Lab Signaling & Dis Res, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai 200092, Peoples R China.,xxie@simm.ac.cn
英文摘要	The direct conversion, or transdifferentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provides promising approaches for cardiac regeneration. However, genetic manipulations raise safety concerns and are thus not desirable in most clinical applications. The discovery of full chemically induced pluripotent stem cells suggest the possibility of replacing transcription factors with chemical cocktails. Here, we report the generation of automatically beating cardiomyocyte-like cells from mouse fibroblasts using only chemical cocktails. These chemical-induced cardiomyocyte-like cells (CiCMs) express cardiomyocyte-specific markers, exhibit sarcomeric organization, and possess typical cardiac calcium flux and electrophysiological features. Genetic lineage tracing confirms the fibroblast origin of these CiCMs. Further studies show the generation of CiCMs passes through a cardiac progenitor stage instead of a pluripotent stage. Bypassing the use of viral-derived factors, this proof of concept study lays a foundation for in vivo cardiac transdifferentiation with pharmacological agents and possibly safer treatment of heart failure.
学科主题	Cell Biology
WOS标题词	Cell Biology
收录类别	SCI
语种	英语
WOS记录号	WOS:000360765600006
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/3932]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Fu, YB,Huang, CW,Xu, XX,et al. Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails[J]. CELL RESEARCH,2015,25(9):1013-1024.
APA	Fu, YB.,Huang, CW.,Xu, XX.,Gu, HF.,Ye, YQ.,...&Xie, X.(2015).Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails.CELL RESEARCH,25(9),1013-1024.
MLA	Fu, YB,et al."Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails".CELL RESEARCH 25.9(2015):1013-1024.