Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins
	Li, Jie 1,2; Chen ZB(陈振斌)1,2,3; Di DL(邸多隆)3
刊名	Food Chemistry
	2012
卷号	132 期号:1 页码:268-276
关键词	Separation Rebaudioside A Stevia Rebaudiana Bertoni Steviosides Macroporous adsorption resin mixed bed
ISSN号	0308-8146
英文摘要	The separation and purification of Rebaudioside A (RA) from Stevia Rebaudiana Bertoni crude extracts (Steviosides) by macroporous adsorption resin (MAR) mixed bed were systematically investigated. MAR mixed bed of HPD750-LSA40-LSA30-DS401 was selected due to its better separation degree. Based on the kinetics/thermodynamics experiment of the mixed bed, it was found that the experimental data fitted better to the pseudo-second-order model, and intra-particle diffusion was rate-limiting step. The adsorption isotherm was consistent with IV equilibrium adsorption isotherm classified by Brunauer. Furthermore, the influencing factors for the separation of RA based on HPLC were also investigated. Under the optimal conditions, the separation degree for RA (D-AS) increased from 0.771 to 1.54. Moreover, the experimental results showed that the purity of the obtained product increased from 60% to 97%.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the Graduate Tutor Program Funds of the Gansu Provincial Department of Education (Grant No. 1001ZSB002)
语种	英语
WOS记录号	WOS:000300465100035
公开日期	2016-02-26
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/19340]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位	1.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China 3.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Jie,Chen ZB,Di DL. Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins[J]. Food Chemistry,2012,132(1):268-276.
APA	Li, Jie,Chen ZB,&Di DL.(2012).Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins.Food Chemistry,132(1),268-276.
MLA	Li, Jie,et al."Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins".Food Chemistry 132.1(2012):268-276.