Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil | |
Zhou, Hangcheng1; Chen, Jixiang1; Yang, Zhi1; Qin B(秦波)2; Li, Yanlin1; Kong, Xiuqin1 | |
刊名 | Annals of Microbiology |
2015 | |
卷号 | 65期号:4页码:2255-2264 |
关键词 | Biosurfactant Bacillus sp. Surface tension Growth kinetics Stability |
ISSN号 | 1590-4261 |
通讯作者 | Chen, Jixiang |
英文摘要 | An effective biosurfactant-producing strain, denoted ZG0427, was isolated from oil-contaminated soil samples collected from an oilfield in northwest China using the drop-collapse test, oil displacement test, and surface tension measurements. The strain was identified as Bacillus sp. based on the results of morphological and biochemical tests and 16S rDNA sequence analysis. The kinetics of cell growth and biosurfactant production were monitored. A maximum biosurfactant production of 2.79 g/L was achieved when the cells were cultivated in fermentation medium containing 2 % (w/v) of glucose as the sole carbon source, with shaking (180 rpm) at 37 °C for 32 h. The extracted biosurfactant reduced the surface tension of deionized water to 24.6 mN/m, with a critical micelle concentration of 50 mg/L, and showed a remarkable ability to reduce both surface tension and emulsification over wide ranges of pH (5.0–12.0), temperature (−70 to 121 °C), and NaCl concentrations (0–10 %, w/v), respectively. The biosurfactant was extracted from the culture supernatant and partially characterized as lipopeptide. |
学科主题 | 分析化学与药物化学 |
收录类别 | SCI |
资助信息 | the National Natural Science Foundation of China (31272694) |
语种 | 英语 |
WOS记录号 | WOS:000364945100046 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/19081] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
作者单位 | 1.Lanzhou Univ Technol, Sch Petrochem Engn, Lanzhou 730050, Gansu, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China |
推荐引用方式 GB/T 7714 | Zhou, Hangcheng,Chen, Jixiang,Yang, Zhi,et al. Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil[J]. Annals of Microbiology,2015,65(4):2255-2264. |
APA | Zhou, Hangcheng,Chen, Jixiang,Yang, Zhi,Qin B,Li, Yanlin,&Kong, Xiuqin.(2015).Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil.Annals of Microbiology,65(4),2255-2264. |
MLA | Zhou, Hangcheng,et al."Biosurfactant production and characterization of Bacillus sp ZG0427 isolated from oil-contaminated soil".Annals of Microbiology 65.4(2015):2255-2264. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论