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	Direct amino acid analysis method for speciation of selenoamino acids using high-performance anion-exchange chromatography coupled with integrated pulsed amperometric detection
	Liang, L; Mo, SM; Cai, YQ; Mou, SF; Jiang, GB; Wen, MJ
	2006
会议名称	18th International Ion Chromatography Symposium
会议日期	SEP 18-21, 2005
会议地点	Montreal, CANADA
关键词	selenoamino acids HPAEC IPAD
英文摘要	Speciation analysis of selenomethylcysteine (SeMeCys), selenomethionine (SeMet) and selenocystine (SeCys) has been performed using a direct amino acid analysis method with high-performance anion-exchange chromatography (HPAEC) coupled with integrated pulsed amperometric detection (IPAD). Three selenoamino acids could be baseline-separated from 19 amino acids using gradient elution conditions for amino acids and determined under new six-potential waveform. Detection limits for SeMeCys, SeMet and SeCys were 0.25, 1 and 20 mu g/L (25 mu L injection, 10 times of the baseline noise), respectively. The relative standard deviations (RSDs) of 200 mu g/L SeMeCys, SeMet and SeCys were 3.1, 4.1 and 2.8%, respectively (n = 9, 25 mu L injection). The proposed method has been applied for determination of selenoamino acids in extracts of garlic and selenious yeast granule samples. No selenoamino acids were found in garlic. Both SeMet and SeCys were detected in selenious yeast tablet with the content of 45 and 129 mu g Se/g, respectively. Selenoamino acids standards were spiked in garlic and yeast granule samples and the recovery ranged from 90 to 106%. (c) 2006 Elsevier B.V. All rights reserved.
ISSN号	0021-9673
WOS记录号	WOS:000238522500022
内容类型	会议论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24744]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liang, L,Mo, SM,Cai, YQ,et al. Direct amino acid analysis method for speciation of selenoamino acids using high-performance anion-exchange chromatography coupled with integrated pulsed amperometric detection[C]. 见:18th International Ion Chromatography Symposium. Montreal, CANADA. SEP 18-21, 2005.

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