Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification

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	Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification
	Hung, Ivan 1; Ge, Yuwei 1,2; Liu, Xiaoli 2; Liu, Mali 2; Li, Conggang 2; Gan, Zhehong 1
刊名	SOLID STATE NUCLEAR MAGNETIC RESONANCE
	2015-11-01
卷号	72 页码:96-103
关键词	Chemical shift anisotropy CSA Magic-angle spinning MAS Spinning sidebands Magic-angle turning MAT CSA amplification Extended chemical shift modulation Protein GB1
英文摘要	Extended chemical shift anisotropy amplification (xCSA) is applied for measuring C-13/N-15 chemical shift anisotropy (CSA) of uniformly labeled proteins under magic-angle spinning (MAS). The amplification sequence consists of a sequence of R-pulses that repetitively interrupt MAS averaging of the CSA interaction. The timing of the pulses is designed to generate amplified spinning sideband manifolds which can be fitted to extract CSA parameters. The C-13/N-15 homonuclear dipolar interactions are not affected by the R-pulses due to the bilinear nature of the spin operators and are averaged by MAS in the xCSA experiment. These features make the constant evolution-time experiment suitable for measuring CSA of uniformly labeled samples. The incorporation of xCSA with multi-dimensional C-13/N-15 correlation is demonstrated with a GB1 protein sample as a model system for measuring C-13/N-15 CSA of all backbone (NH)-N-15, (13)CA and (CO)-C-13 sites. (C) 2015 Published by Elsevier Inc.
WOS标题词	Science & Technology ; Physical Sciences ; Technology
类目[WOS]	Chemistry, Physical ; Physics, Atomic, Molecular & Chemical ; Physics, Condensed Matter ; Spectroscopy
研究领域[WOS]	Chemistry ; Physics ; Spectroscopy
关键词[WOS]	SOLID-STATE NMR ; NUCLEAR-MAGNETIC-RESONANCE ; ANGLE-SPINNING NMR ; MAGIC-ANGLE ; MICROCRYSTALLINE PROTEIN ; SPECTROSCOPY ; TENSORS ; SEQUENCES ; SPECTRA ; SAMPLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000365914000012
公开日期	2016-01-19
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/9075]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Natl High Magnet Field Lab, Ctr Interdisciplinary Magnet Resonance, Tallahassee, FL 32310 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Peoples R China
推荐引用方式 GB/T 7714	Hung, Ivan,Ge, Yuwei,Liu, Xiaoli,et al. Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification[J]. SOLID STATE NUCLEAR MAGNETIC RESONANCE,2015,72:96-103.
APA	Hung, Ivan,Ge, Yuwei,Liu, Xiaoli,Liu, Mali,Li, Conggang,&Gan, Zhehong.(2015).Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification.SOLID STATE NUCLEAR MAGNETIC RESONANCE,72,96-103.
MLA	Hung, Ivan,et al."Measuring C-13/N-15 chemical shift anisotropy in [C-13,N-15] uniformly enriched proteins using CSA amplification".SOLID STATE NUCLEAR MAGNETIC RESONANCE 72(2015):96-103.