Folic acid deficiency impairs the gill health status associated with the NF-kappa B, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Folic acid deficiency impairs the gill health status associated with the NF-kappa B, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella)
	Shi, Lei 1; Feng, Lin 1,2,3; Jiang, Wei-Dan 1,2,3; Liu, Yang 1,2,3; Jiang, Jun 1,2,3; Wu, Pei 1,2,3; Zhao, Juan 1; Kuang, Sheng-Yao 4; Tang, Ling 4; Tang, Wu-Neng 4
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-11-01
卷号	47 期号:1 页码:289-301
关键词	Folic acid Grass carp Gills Immune function Barrier function
ISSN号	1050-4648
英文摘要	The aim of this study was to investigate the effect of dietary folic acid on fish growth, the immune and barrier functions of fish gills, and the potential mechanisms of these effects. Young grass carp (Ctenophatyngodon idella) were fed diets containing graded levels of folic acid at 0.10 (basal diet), 0.47, 1.03, 1.48, 1.88 and 3.12 mg kg(-1) diet for 8 weeks. The results showed that acid phosphatase and lysozyme activities and the complement component 3 content in fish gills decreased with folic acid deficiency (P < 0.05). Folic acid deficiency up-regulated liver-expressed antimicrobial peptide 1, interleukin 1 beta, interleukin 8, tumor necrosis factor alpha, nuclear factor kappa B p65, I kappa B kinase alpha (IKK-alpha), IKK-beta and IKK-gamma gene expression. Folic acid deficiency down-regulated interleukin 10, transforming growth factor beta, I kappa B and target of rapamycin gene expression in fish gills (P < 0.05). These results showed that limited folic acid decreased fish gill immune status. Furthermore, folic acid deficiency down-regulated claudin-b, claudin-c, claudin-3, occludin and zonula occludens 1 gene expression, whereas folic acid deficiency up-regulated claudin-12, claudin-15, myosin light chain kinase and p38 mitogen activated protein kinase gene expression in fish gills (P < 0.05). These results suggested that folic acid deficiency disrupted tight junction-mediated fish gill barrier function. Additionally, folic acid deficiency increased the content of reactive oxygen species, protein carbonyl and malondialdehyde (MDA); Mn superoxide dismutase activity and gene expression; and Kelch-like-ECH-associated protein 1a (Keap1a) and Keap1b gene expression (P < 0.05). Conversely, folic acid deficiency decreased Cu/Zn superoxide dismutase, catalase, glutathione peroxidase, glutathione s-transferases and glutathione reductase activities and gene expression as well as NF-E2-related factor 2 gene expression in fish gills (P < 0.05). All of these results indicated that folic acid deficiency impaired fish gill health status via regulating gene expression of cytokines, tight junction proteins, antioxidant enzymes, NF-kappa B p65, MLCK and Nrf2. Based on percent weight gain, LZ activity and MDA content in the gills, the dietary folic acid requirements for young grass carp were 1.60, 2.07 and 2.08 mg kg(-1), respectively. (C) 2015 Published by Elsevier Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	TIGHT JUNCTION PROTEINS ; C-REACTIVE PROTEIN ; NONSPECIFIC IMMUNE-RESPONSES ; INTESTINAL MUCOSAL IMMUNE ; MESSENGER-RNA EXPRESSION ; GENE-EXPRESSION ; ANTIOXIDANT CAPACITY ; GROWTH-PERFORMANCE ; ENDOTHELIAL-CELLS ; ENZYME-ACTIVITIES
收录类别	SCI
语种	英语
WOS记录号	WOS:000365053300034
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/27404]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Shi, Lei,Feng, Lin,Jiang, Wei-Dan,et al. Folic acid deficiency impairs the gill health status associated with the NF-kappa B, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,47(1):289-301.
APA	Shi, Lei.,Feng, Lin.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,...&Zhou, Xiao-Qiu.(2015).Folic acid deficiency impairs the gill health status associated with the NF-kappa B, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,47(1),289-301.
MLA	Shi, Lei,et al."Folic acid deficiency impairs the gill health status associated with the NF-kappa B, MLCK and Nrf2 signaling pathways in the gills of young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 47.1(2015):289-301.