Arabidopsis VQ motif-containing proteins VQ12 and VQ29 negatively modulate basal defense against Botrytis cinerea

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 植物分子生物学研究组

	Arabidopsis VQ motif-containing proteins VQ12 and VQ29 negatively modulate basal defense against Botrytis cinerea
	Yu, Diqiu; Hu, Yanru
刊名	SCIENTIFIC REPORTS
	2015
卷号	5 页码:-
ISSN号	2045-2322
中文摘要	Arabidopsis VQ motif-containing proteins have recently been demonstrated to interact with several WRKY transcription factors; however, their specific biological functions and the molecular mechanisms underlying their involvement in defense responses remain largely unclear. Here, we showed that two VQ genes, VQ12 and VQ29, were highly responsive to the necrotrophic fungal pathogen Botrytis cinerea. To characterize their roles in plant defense, we generated amiR-vq12 transgenic plants by using an artificial miRNA approach to suppress the expression of VQ12, and isolated a loss-of-function mutant of VQ29. Phenotypic analysis showed that decreasing the expression of VQ12 and VQ29 simultaneously rendered the amiR-vq12 vq29 double mutant plants resistant against B. cinerea. Consistently, the B. cinerea-induced expression of defense-related PLANT DEFENSIN1.2 (PDF1.2) was increased in amiR-vq12 vq29. In contrast, constitutively-expressing VQ12 or VQ29 confered transgenic plants susceptible to B. cinerea. Further investigation revealed that VQ12 and VQ29 physically interacted with themselves and each other to form homodimers and heterodimer. Moreover, expression analysis of VQ12 and VQ29 in defense-signaling mutants suggested that they were partially involved in jasmonate (JA)-signaling pathway. Taken together, our study indicates that VQ12 and VQ29 negatively regulate plant basal resistance against B. cinerea.
资助信息	Natural Science Foundation of China [31401040]; Youth Innovation Promotion Association CAS; CAS "Light of West China" Program; Yunnan provincial government [2014HC017]
原文出处	10.1038/srep14185
语种	英语
公开日期	2015-11-19
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/9357]
专题	西双版纳热带植物园_植物分子生物学研究组西双版纳热带植物园_2012年后新成立研究组
推荐引用方式 GB/T 7714	Yu, Diqiu,Hu, Yanru. Arabidopsis VQ motif-containing proteins VQ12 and VQ29 negatively modulate basal defense against Botrytis cinerea[J]. SCIENTIFIC REPORTS,2015,5:-.
APA	Yu, Diqiu,&Hu, Yanru.(2015).Arabidopsis VQ motif-containing proteins VQ12 and VQ29 negatively modulate basal defense against Botrytis cinerea.SCIENTIFIC REPORTS,5,-.
MLA	Yu, Diqiu,et al."Arabidopsis VQ motif-containing proteins VQ12 and VQ29 negatively modulate basal defense against Botrytis cinerea".SCIENTIFIC REPORTS 5(2015):-.