A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures

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	A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures
	Zeng, DD; Zhang, H; Zhu, D; Li, J; San, LL; Wang, ZH; Wang, CG; Wang, YS; Wang, LH; Zuo, XL
刊名	BIOSENSORS & BIOELECTRONICS
	2015
卷号	71 页码:434—438
关键词	SEQUENCE-SPECIFIC DETECTION AMPLIFICATION HYBRIDIZATION BIOSENSOR
英文摘要	Electrochemical DNA (E-DNA) sensor is an important tool for detecting DNA biomarker. In this work, we have demonstrated a novel strategy of E-DNA sensor based on DNA tetrahedral nanostructures for the sensitive detection of target DNA. In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively. The biotin-tagged three dimensional DNA tetrahedral nanostructures were employed for efficient signal amplification by capturing multiple catalytic enzymes. Such improved E-DNA sensor can sensitively detect DNA target as low as 1 fM with excellent differentiation ability for even single mismatch. And a mean recovery rate of 90.57% in DNA solution extracted from human serum was obtained. We have also compared this new method of attaching catalytic enzymes with the other two typical methods: One is through biotinylated single-stranded DNA (SSDNA) and the other is through gold nanoparticles (GNPs). Results indicated that the RTSPs-based enzyme amplification system showed much better performance than the other two systems. (C) 2015 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/24381]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Zeng, DD,Zhang, H,Zhu, D,et al. A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures[J]. BIOSENSORS & BIOELECTRONICS,2015,71:434—438.
APA	Zeng, DD.,Zhang, H.,Zhu, D.,Li, J.,San, LL.,...&Mi, XQ.(2015).A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures.BIOSENSORS & BIOELECTRONICS,71,434—438.
MLA	Zeng, DD,et al."A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures".BIOSENSORS & BIOELECTRONICS 71(2015):434—438.