Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities | |
Zhou XJ(周贤婧)1,2; Chen J(陈娟)1; Shi YP(师彦平)1 | |
刊名 | Cogent Chemistry |
2015 | |
卷号 | 1页码:1100527(1-10) |
关键词 | polyphenols honey capillary electrophoresis DPPH potential marker |
ISSN号 | 2331-2009 |
通讯作者 | 师彦平 |
英文摘要 | A micellar electrokinetic chromatography method was developed to quantify the levels of four main polyphenols in various floral sources honeys. The effect of several parameters—such as pH, sodium dodecyl sulfate (SDS) of the buffer, separation voltage and injection time—were systematically investigated. The main polyphenols were successfully separated within 13 min of UV detection at 214 nm. In the tested concentration range, regression equations revealed good linear relationships between the peak areas and corresponding concentration (correlation coefficients ranged from 0.9984 to 0.9997). Moreover, the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activities were also quantified. These compounds are not present in large amounts, but can reach up to 2758 μg in 100 g−1 of honey, which are the main flavonoid aglycones found in honey. The flavonoid glycosides are hydrolyzed by bee enzymes to render the aglycones. Therefore, the flavonoid aglycones should be served as a potential marker and capillary electrophoresis could be used as an effective way to look for in various floral sources. |
学科主题 | 分析化学与药物化学 |
资助信息 | the National Key Technology Research and Development Program of China [grant number 2011BAI05B02];the National Natural Science Foundation of China [grant number 21105106];[grant number 21375136] |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://210.77.64.217/handle/362003/18982] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
作者单位 | 1.Key Laboratory of Chemistry of Northwestern Plant Resources of the CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China 2.University of Chinese Academy of Sciences, Beijing 100039, P.R. China |
推荐引用方式 GB/T 7714 | Zhou XJ,Chen J,Shi YP. Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities[J]. Cogent Chemistry,2015,1:1100527(1-10). |
APA | Zhou XJ,Chen J,&Shi YP.(2015).Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities.Cogent Chemistry,1,1100527(1-10). |
MLA | Zhou XJ,et al."Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities".Cogent Chemistry 1(2015):1100527(1-10). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论