Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities
Zhou XJ(周贤婧)1,2; Chen J(陈娟)1; Shi YP(师彦平)1
刊名Cogent Chemistry
2015
卷号1页码:1100527(1-10)
关键词polyphenols honey capillary electrophoresis DPPH potential marker
ISSN号2331-2009
通讯作者师彦平
英文摘要A micellar electrokinetic chromatography method was developed to quantify the levels of four main polyphenols in various floral sources honeys. The effect of several parameters—such as pH, sodium dodecyl sulfate (SDS) of the buffer, separation voltage and injection time—were systematically investigated. The main polyphenols were successfully separated within 13 min of UV detection at 214 nm. In the tested concentration range, regression equations revealed good linear relationships between the peak areas and corresponding concentration (correlation coefficients ranged from 0.9984 to 0.9997). Moreover, the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical-scavenging activities were also quantified. These compounds are not present in large amounts, but can reach up to 2758 μg in 100 g−1 of honey, which are the main flavonoid aglycones found in honey. The flavonoid glycosides are hydrolyzed by bee enzymes to render the aglycones. Therefore, the flavonoid aglycones should be served as a potential marker and capillary electrophoresis could be used as an effective way to look for in various floral sources.
学科主题分析化学与药物化学
资助信息the National Key Technology Research and Development Program of China [grant number 2011BAI05B02];the National Natural Science Foundation of China [grant number 21105106];[grant number 21375136]
语种英语
内容类型期刊论文
源URL[http://210.77.64.217/handle/362003/18982]  
专题兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位1.Key Laboratory of Chemistry of Northwestern Plant Resources of the CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R. China
2.University of Chinese Academy of Sciences, Beijing 100039, P.R. China
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GB/T 7714
Zhou XJ,Chen J,Shi YP. Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities[J]. Cogent Chemistry,2015,1:1100527(1-10).
APA Zhou XJ,Chen J,&Shi YP.(2015).Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities.Cogent Chemistry,1,1100527(1-10).
MLA Zhou XJ,et al."Rapid and sensitive determination of polyphenols composition of unifloral honey samples with their antioxidant capacities".Cogent Chemistry 1(2015):1100527(1-10).
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