Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry
	Sun YP(孙英佩)1,2,3; Chen J(陈娟)1,2 ; Qi HY(戚欢阳)1,2 ; Shi YP(师彦平)1,2 ; Qi HY(戚欢阳); Shi YP(师彦平)
刊名	Journal of Chromatography B
	2015
卷号	1004 页码:53-59
关键词	Uric acid (UA) Graphitic carbon nitrides (g-CNs) Hollow fiber (HF) Solid phase microextraction (SPME) Gas chromatography-mass spectrometry (GC/MS)
ISSN号	1570-0232
通讯作者	戚欢阳 ; 师彦平
英文摘要	An elevated uric acid (UA) in urine or serum can affect renal function and blood pressure, which is an indicator of gout, cardiovascular and renal diseases, hypertension, etc. In this work, a new type of mixed matrix membrane (MMM), based on graphitic carbon nitrides (g-CNs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine UA in urine and serum followed by gas chromatography-mass spectrometry (GC/MS). The porous g-CNs were dispersed in ammonia, and then the exfoliated g-CNs nanosheets were held in the pores of HF by capillary forces and sonification. The prepared g-CNs modified HF (g-CNs-HF) was immersed in biofluid directly to extract UA with SPME mode and the solvent-free mode is convenient for further derivatization and analysis. To achieve the highest extraction efficiency (EF), main extraction and derivatization parameters, such as g-CNs-HF immobilizing time, sonification power and time of extraction, derivatization and desorption time, were optimized. Under the optimum extraction conditions, a favorable linearity of UA was obtained in the range 0.1-200 mu g mL(-1) with correlation coefficients higher than 0.9990, and the average recoveries at three spiked levels of UA in urine and serum ranged from 80.7% to 121.6%, from 84.7% to 101.1%, respectively. The obtained results demonstrated the developed g-CNs-HF-SPME is a simple, rapid, cost-effective, solvent-free method for the analysis of UA in biofluid.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Nature Science Foundation of China (Nos. 21375136;21105106);the Scientific research project of Central Asia Drug Discovery and Development Centre of Chinese Academy of Sciences (No. CAM201404)
语种	英语
WOS记录号	WOS:000364246300008
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/18978]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Qi HY(戚欢阳); Shi YP(师彦平)
作者单位	1.Chinese Acad Sci, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Sun YP,Chen J,Qi HY,et al. Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry[J]. Journal of Chromatography B,2015,1004:53-59.
APA	Sun YP,Chen J,Qi HY,Shi YP,戚欢阳,&师彦平.(2015).Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry.Journal of Chromatography B,1004,53-59.
MLA	Sun YP,et al."Graphitic carbon nitrides modified hollow fiber solid phase microextraction for extraction and determination of uric acid in urine and serum coupled with gas chromatography-mass spectrometry".Journal of Chromatography B 1004(2015):53-59.