Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities
	Zeng LM(曾黎明)1,2,3; Jin H(金辉)1,2,4 ; Lu DX(路等学)5; Yang XY(杨晓燕)1,2 ; Pan L(潘乐)1,2,3; Cui HY(崔海燕)1,2,3; He XF(何小凤)1,2; Qiu HD(邱洪灯)1,2 ; Qin B(秦波)1,2 ; Qin B(秦波)
刊名	Journal of Basic Microbiology
	2015
卷号	55 期号:10 页码:1239-1244
关键词	Bacillus sp SMrs28 Novel compound Secondary metabolites Nematicidal activity
ISSN号	0233-111X
通讯作者	秦波
英文摘要	A strain SMrs28 was isolated from the rhizosphere soil of a toxic plant Stellera chamaejasme and identified as Bacillus sp. on the basis of morphological and partial 16S rRNA gene sequence analysis. The crude extract of SMrs28 fermentation broth showed strong nematocidal activities in preliminary test. To define the active nematocidal metabolites of SMrs28, a novel compound (1), 4-oxabicyclo[3.2.2]nona-1(7), 5,8-triene, along with five known compounds (2-6), were isolated from the strain by various column chromatographic techniques and characterized on the basis of spectroscopic analysis. Results of the in vitro nematicidal tests showed that the metabolites presented different levels of activity at certain exposure conditions. Compounds (1-3) displayed LC50 values of 904.12, 451.26, 232.98 Rg/m1 and 1594.0, 366.62, 206.38 mu g/ml against Bursaphelenchus xylophilus and Ditylenchus destructor at 72 h, respectively. This is the first report of the nematicidal activity of the compounds as constituents of Bacillus sp.. Our findings help to find potential chemical structures to develop nematicides from microbial source for the management of nematode-infected plant diseases.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	National Natural Science Foundation of China (grant no. 31070386);Basic Research Program of Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (080423SYR1);the associate scholar program for talents cultivation plan of “Western Light” of Chinese Academy of Sciences;the Province-academy Cooperation Program of Henan Province of China (grant no. 102106000021);the open project of Key Laboratory of Tobacco Diseases and Insect Pests Monitoring Controlling and Integrated Management, Institute of Tobacco Research, Chinese Academy of Agricultural Sciences (IPM 201404)
语种	英语
WOS记录号	WOS:000363891600010
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/18963]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Qin B(秦波); Qin B(秦波); Qin B(秦波)
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources CAS, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Agr Sci, Inst Tobacco Res, Key Lab Tobacco Dis & Insect Pests Monitoring Con, Qingdao, Peoples R China 5.Gansu Acad Sci, Inst Biol, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Zeng LM,Jin H,Lu DX,et al. Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities[J]. Journal of Basic Microbiology,2015,55(10):1239-1244.
APA	Zeng LM.,Jin H.,Lu DX.,Yang XY.,Pan L.,...&秦波.(2015).Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities.Journal of Basic Microbiology,55(10),1239-1244.
MLA	Zeng LM,et al."Isolation and identification of chemical constituents from the bacterium Bacillus sp and their nematicidal activities".Journal of Basic Microbiology 55.10(2015):1239-1244.