Application of dimethyl-beta-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomerseparation of ephedrine and related compounds in some drugs

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Application of dimethyl-beta-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomerseparation of ephedrine and related compounds in some drugs
	Liu, LB; Zheng, ZX; Lin, JM
刊名	BIOMEDICAL CHROMATOGRAPHY
	2005
卷号	19 期号:6 页码:447-453
关键词	capillary electrophoresis chiral separation dimethyl-beta-CD ephedrine
英文摘要	Dimethyl-beta-cyclodextrin (DM-beta-CD) modified capillary electrophoresishas been developed for chiral separation of ephedrine and related compounds, such as (+/-)-norephedrine, (+/-)-N-methylephedrine, (+/-)-ephedrine and (+)-pseudoephedrine. The influence of some crucial parameters such as buffer concentration, pH value, DM-beta-CD concentration, applied voltage and separation temperature on the separation was investigated. Under the optimum conditions, i.e. 40 mm DM-beta-CD in 75 mm Tris (pH 2.5) as the running electrolyte, separation voltage +25 kV and temperature 25 degrees C, a satisfactory separation of the enantiomers was accomplished. The detection limits (S/N = 3) ranged from 65 to 161 ng/mL and the linear range was 0.15 to 101.0 mu g/mL for pressure injection. The present method was successfully applied for the analysis of a series of drugs such as anti-tussive, the drug for rheum, the drug for rhinitis and a Chinese traditional herbal medicine, Ephedrae herba (Ma-Huang in Chinese). The recoveries of ephedrine and related compounds in real samples ranged from 97.6 to 103.5%. This method is useful in the simple and rapid analysis of ephedrine derivatives in marketed products. Copyright (C) 2005 John Wiley,& Sons, Ltd.
学科主题	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/23141]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, LB,Zheng, ZX,Lin, JM. Application of dimethyl-beta-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomerseparation of ephedrine and related compounds in some drugs[J]. BIOMEDICAL CHROMATOGRAPHY,2005,19(6):447-453.
APA	Liu, LB,Zheng, ZX,&Lin, JM.(2005).Application of dimethyl-beta-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomerseparation of ephedrine and related compounds in some drugs.BIOMEDICAL CHROMATOGRAPHY,19(6),447-453.
MLA	Liu, LB,et al."Application of dimethyl-beta-cyclodextrin as a chiral selector in capillary electrophoresis for enantiomerseparation of ephedrine and related compounds in some drugs".BIOMEDICAL CHROMATOGRAPHY 19.6(2005):447-453.