R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time
	Chai, Guohua 1; Wang, Zengguang 1; Tang, Xianfeng 1; Yu, Li 1; Qi, Guang 1; Wang, Dian 1; Yan, Xiaofei 1; Kong, Yingzhen 2; Zhou, Gongke 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2014-08-01
卷号	65 期号:15 页码:4255-4269
关键词	Arabidopsis evolution flowering time gene pair Populus R2R3-MYB secondary wall thickening
英文摘要	In plants, the R2R3-MYB gene family contains many pairs of paralogous genes, which play the diverse roles in developmental processes and environmental responses. The paper reports the characterization of 81 pairs of Populus R2R3-MYB genes. Chromosome placement, phylogenetic, and motif structure analyses showed that these gene pairs resulted from multiple types of gene duplications and had five different gene fates. Tissue expression patterns revealed that most duplicated genes were specifically expressed in the tissues examined. qRT-PCR confirmed that nine pairs were highly expressed in xylem, of which three pairs (PdMYB10/128, PdMYB90/167, and PdMYB92/125) were further functionally characterized. The six PdMYBs were localized to the nucleus and had transcriptional activities in yeast. The heterologous expression of PdMYB10 and 128 in Arabidopsis increased stem fibre cell-wall thickness and delayed flowering. In contrast, overexpression of PdMYB90, 167, 92, and 125 in Arabidopsis decreased stem fibre and vessel cell-wall thickness and promoted flowering. Cellulose, xylose, and lignin contents were changed in overexpression plants. The expression levels of several genes involved in secondary wall formation and flowering were affected by the overexpression of the six PdMYBs in Arabidopsis. This study addresses the diversity of gene duplications in Populus R2R3-MYBs and the roles of these six genes in secondary wall formation and flowering control.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	MYB TRANSCRIPTION FACTORS ; ARABIDOPSIS-THALIANA ; ABIOTIC STRESS ; POPLAR ; FAMILY ; CELL ; BIOSYNTHESIS ; EXPRESSION ; PROTEIN ; TRICHOCARPA
收录类别	SCI
语种	英语
WOS记录号	WOS:000339954300012
公开日期	2015-12-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/6257]
专题	青岛生物能源与过程研究所_植物代谢工程团队
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resource, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Chai, Guohua,Wang, Zengguang,Tang, Xianfeng,et al. R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(15):4255-4269.
APA	Chai, Guohua.,Wang, Zengguang.,Tang, Xianfeng.,Yu, Li.,Qi, Guang.,...&Zhou, Gongke.(2014).R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time.JOURNAL OF EXPERIMENTAL BOTANY,65(15),4255-4269.
MLA	Chai, Guohua,et al."R2R3-MYB gene pairs in Populus: evolution and contribution to secondary wall formation and flowering time".JOURNAL OF EXPERIMENTAL BOTANY 65.15(2014):4255-4269.